Cancer is a group of diseases characterized by unregulated cell growth including the invasion and spread of mutated cells from the site of origin. Chemotherapy is a medicine which is used to kill cancer cells by damaging them so they can’t reproduce and spread. Chemotherapy also kills healthy living cells, which causes unwanted side effects such as hair loss, tiredness and pale skin.
In this experiment we used MTT Assay to analyse which drug is more effective in killing cancer cells and keeps healthy cells viable. Our interest in this fundamental biomedical question stems from, the use of chemotherapy, and to investigate how we can reduce the damage to healthy living cells, while still using chemotherapy as a treatment for cancer.
Controls as used in majority of experiments, they were used to identify spectrophotometric wavelengths (SW) between cancer cells (CC) and healthy living (HL) cells. These were used in comparison with serial dilutions containing …show more content…
From looking at the results in Fig.2 from control (HL) we obtained a wavelength of 0.835nm in comparison with the (CC) which had a wavelength of 0.908nm. This provides evidence that darker regent less wavelength of light is able to pass through the solutions, therefore supports the theory that more cells were at a normal and functioning level. Lower the wavelength, indicates more cells are active and are functioning efficiently. In addition to this, we came to conclusion that drug A is the most effective treatment for the use of chemotherapy, based on observations from this experiment. This is because both drug A and B kill the same number of cancer cells, as they both have absorbance’s at 0.688nm. Overall drug A is more effective in maintaining healthy living cells, compared to drug B. This technique was shown to be an effective way of analysing that drug A is most effective in maintain healthy cells while killing cancerous
In this experiment we used MTT Assay to analyse which drug is more effective in killing cancer cells and keeps healthy cells viable. Our interest in this fundamental biomedical question stems from, the use of chemotherapy, and to investigate how we can reduce the damage to healthy living cells, while still using chemotherapy as a treatment for cancer.
Controls as used in majority of experiments, they were used to identify spectrophotometric wavelengths (SW) between cancer cells (CC) and healthy living (HL) cells. These were used in comparison with serial dilutions containing …show more content…
From looking at the results in Fig.2 from control (HL) we obtained a wavelength of 0.835nm in comparison with the (CC) which had a wavelength of 0.908nm. This provides evidence that darker regent less wavelength of light is able to pass through the solutions, therefore supports the theory that more cells were at a normal and functioning level. Lower the wavelength, indicates more cells are active and are functioning efficiently. In addition to this, we came to conclusion that drug A is the most effective treatment for the use of chemotherapy, based on observations from this experiment. This is because both drug A and B kill the same number of cancer cells, as they both have absorbance’s at 0.688nm. Overall drug A is more effective in maintaining healthy living cells, compared to drug B. This technique was shown to be an effective way of analysing that drug A is most effective in maintain healthy cells while killing cancerous