Why Crp Has Been Proven For Patients With Acute Pneumococcal Pneumonia At The Rockefeller Institute Of Medical Research

1744 Words Sep 8th, 2015 7 Pages
CRP was identified from patients with acute pneumococcal pneumonia at the Rockefeller Institute for Medical Research (Tillett and Francis, 1930), a name that reflects its reaction with the C-polysaccharide of Pneumococcus. This pentameric protein with MW 118 kDa has five non-covalently bonded and non-glycosylated identical subunits of 206 amino acids each to form a disk-shaped pentagon (Fig. 1). CRP has been proven as an early indicator of infectious or inflammatory conditions as well as a universal biomarker for a wide range of diseases and disorders. As such diseases often commence at very low CRP levels, nM or even pM, analytical methods must be highly sensitive, selective, fast and reliable using minimum sample volume. Therefore, sensitive analysis of CRP levels in biological samples with these requirements is of critical importance for diagnosis and monitoring the progress of chemotherapy/personalized healthcare intervention. Although monomeric CRP exhibits different binding capacities to complementary proteins from pentameric CRP, there is still problematic to analyze these two forms by antibody (Ab)-based assays.
In brief, most advanced formats have just emerged in the last three years and originated with turbidimetry based assays to detect CRP in µg mL-1, followed by more sensitive ELISA, chemiluminescent, fluorescent and electrochemical assays with detection sensitivity down to fg mL-1. In concert with significant advances in microfluidics, lab-on-a-chip (LOC) and…

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