State the optimum pH for sucrase activity and describe how sucrase activity changes at more acidic and more alkaline pH values. Table 2: Effect of Temperature on Sucrase Activity Optical Density 10 °CC (50 °F) 20 °C (68 °F) 30 °C (86 °F) 40 °C (104 °F) 50 °C (122 °F) 60 °C (140 °F) 70 °C (158 °F) 1 0.006 0.273 0.791 0.940 0.927 0.807 0.613 2 0.010 0.285 0.761 0.954 0.934 0.846 0.604 3 0.009 0.255 0.773 0.941 0.907 0.845 0.642 average 0.008 0.271 0.775 0.945 0.923 0.833 0.620 Effect of Temperature on Sucrase Activity 2. Was the rate of increase of sucrase activity higher at a pH of 8.5 or a pH of 5.5?…
What is the ideal pH for its functionality? Is the activity of the enzyme affected by the temperature? Is tyrosinase identify by using electrophoresis? Hypothesis for the experiments are as follow: if tyrosinase is place in a pH of 6 will it continue to function normally and will light be absorbed at 475nm. If tyrosinase is put in a pH of 4 or 8 can the function be hindered if it’s not arrested completely?…
When high temperatures are reach the hydrogen bonds break and the 3D shape is changed. When boiling the enzyme or extract, such high temperature result in a permanent change in the structure of the protein called denaturation (Dolphin, 2015). Competitive inhibitors can affect how easily the substrate can bind to the enzyme. Hypothesis for effect of temperature: As the temperatures increase the enzyme and substrate will collide at a faster rate causing the hydrogen bonds to break and the shape of the enzyme to change. Hypothesis for effect of pH: The enzyme reaction rate of binding to the substrate will favor a more neutral pH value so that the shape of the enzyme is not altered.…
In most cases, a chemical reaction is able to occur in more than one way; enzyme kinetics studies the effect of how biological catalysts can influence biochemical reactions. Enzymes play an important role in regulating metabolic pathways in the human body and without enzymes they will not function efficiently. The rate of a reaction is enhanced by enzyme activity which provides alternate pathways for the reaction to proceed by.…
Our cells usually make toxic byproducts however they use enzymes to break them down into harmless chemicals. When an excess amount of H2O2 (hydrogen peroxide) is formed in the cell during metabolism/cell respiration catalase enzymes are utilized to break down the toxic chemical into oxygen gas and water. An experiment was conducted was to see if the Ph level affected the rate of which the catalase broke down hydrogen peroxide. A hypothesis was created, which stated that if the ph was lower than the control than the rate of hydrogen peroxide decomposition would be greater. In the experiment there were three trials, a control, a high level ph, and a low level…
The different jobs that Enzymes do rely on the temperature, ionic conditions, and PH level of the environment. Enzymes work in different environments. For instance, some enzymes function better with acidic PHs while others are better in less acidic, or neutral surroundings. In many organisms, enzymes are extremely important because the various biochemical reactions occurring in the organisms need enzymes to speed up the chemical…
After we did the experiment for pH 4, it can be concluded that the gas level increased because it started at a gas level of 7 and in the end, it was at 34. After the experiment for pH 7, the gas level increased because it started at a gas level of 9 and in the end, it was at 36. After the experiment for pH 10, the gas level increased even more than pH 7 because the gas level started at 10, but in the end, it was at 40. Even though those are the results we got in the lab that is not how it is supposed to be that because pH 7 was supposed to have the highest increased gas levels because this enzyme works best in a neutral pH environment, so it should be pH7 instead of pH…
The hydrogen atoms within the protein bonds disconnect, causing the protein to lose its shape. Thus it ability to intake substrates. Ph plays an important role in the enzyme rate of production due to it’s ability to alter electric charges. If Ph is too high or too…
EXPERIMENT: The effect of an acidic fluid on the activity of an enzyme INTRODUCTION: There are several factors that may influence the activity of an enzyme to include temperature and the pH levels. In order to better understand how the pH level affects the actions of an enzyme, the experiment will introduce different foods with acids and bases through mixture of direct contact. OBJECTIVE:…
The goal of this experiment was to determine the Michaelis constant (Km) and also the maximal velocity (Vmax) and the inhibition of alkaline phosphate. In order to accomplish these goals, 5 samples were used. Each sample contained different volumes of 0.2 m MPNPP (p-nitrophenylphosphate) and 0.2 M Tris-Hcl at a pH of 8.0. To each sample 0.2 mL of the enzyme studied (Alkaline Phosphatase) were added upon insertion on the spectrophotometer apparatus. With intervals of 20 seconds their absorbance at a wavelength of 410 nm was recorded at time frame of 2 minutes for each solution.…
The five drops of hydrogen peroxide and, when applicable, one millimeter of catalase was added to the tube first. We then executed the transformations listed above as independent variables to alter the pH or the temperature, whether it was using an ice bath or adding a base or acid solution. After two minutes had passed, we took a ruler to measure the height of the bubbles and recorded it. To analyze this new data, we compared the different amounts for each trial to deduce how environmental factors affected enzyme activity. This study is a controlled experiment designed to investigate enzymes and practice interpreting…
Escherichia coli, a bacteria with an optimal pH of between 6.5 to 7.0 to enhance its proliferation is very commonly used in laboratories and studies worldwide due to its low cost and high effectiveness and will therefore be the bacteria of choice for this investigation. It has been shown in many studies such as the article “HdeB chaperone activity is coupled to its intrinsic dynamic properties” very recently published in the prestigious magazine Nature that bacterial growth and overall metabolism can be inhibited by alterations in the pH of the culture. The active ingredient hypochlorous acid, found in bleach, affects proteins in bacteria by changing the pH of the environment and therefore denaturing them by affecting their secondary and tertiary structures. Moreover, hypochlorous acid has been found to have the same…
The enzyme reached a maximum at its greatest absorbance point of 0.526 at 10°C, the lowest temperature tested. Enzymes are most active at their ideal temperature, allowing us to confirm that 10°C is the optimal temperature for catechol oxidase. Temperatures that are too high or too low can denature enzyme shape and function. A test was conducted, monitoring a phosphate buffer at several levels of pH. As seen in Figure 2, the phosphate buffer with a neutral pH of 7, had the highest average absorbance level of 0.513. Thus, catechol oxidase has an optimal pH of…
Many factors can affect the enzyme activity (including temperature, pH, substrate concentration), so all conditions apart from the one being quantified should be standardised. The…
Effect of temperature Temperature has a profound influence on the rate of all processes occurring in living organisms including fungi and it may determine the type of reproduction, the morphology of the organism and the nutrients required. For any particular organism, the minimum and maximum temperatures are the lowest and highest at which growth occurs, and the optimum is that at which the growth rate is the greatest. Majority of fungi are mesophylls and grow at moderate temperature. These values are called cardinal values. Temperature range of different enzymes are specific and it varies with different organisms.…