Essay on What Are A Dna Barcode?
DNA barcoding is fast, accurate method of identifying plant and animals, or products made from them. It’s a DNA sequence that uniquely identifies each species of living things by comparing them with known barcodes in large online databases. When you get to the section where the animation separates into plant and animal cells, select animal cells: 2. What are the two steps taken at the beginning of the process to break down the cell membrane? a. Adding lysis solution to tube which dissolves membrane bound organelles b. Twisting a clean pestle against surface and grinding the tissue which breaks up the cell walls and other tough materials
3. What is the first step to separate the cellular debris from the DNA?
DNA is removed from cellular debris then purified. This involves centrifucation to collect cellular debris at bottom of tube.
4. After spinning the sample-do we keep the pellet or the supernatant?
We keep the supernatant.
5. What is the purpose of adding the silica?
Silica is a DNA binding matrix. In the presence of lysis solution the silica binds readily to nucleic acid.
6. What is the purpose of washing the silica pellet?
Wash buffer removes contaminants from sample while nucleic acids remain bound to resin. Silica isn’t soluble in the wash buffer. The silica may stay as pellet or break up during washing.
7. Why do we add water to the pellet in the final step?
To elute the DNA. Add water and incubate to release DNA from silica pellet.