Western Blots

Abstract:
Evolution is an ongoing process, it states that present day organisms arose from preexisting organism. The amino acid sequence of a protein allows scientist to compare the evolutionary relationship among species since proteins have almost the same functions. The greater the difference between organisms means that there is more variance in the amino acid sequencing. However, proteins do most of the cell’s work and they also function as an immune antibody. The immune system consists of diverse cells and antibody molecules. Antibody is blood proteins that get activated when foreign organisms invade the body. They recognize certain antigens based on their epitope, which is usually five to eight amino acids long on the surface of the protein. Western blot is used to visualize the proteins that will respond to specific antiserum after we separate the proteins. Then place the serums against nitrocellulose …show more content…
In western blotting or immunoblotting an antibody is used to specifically detect its antigen. The interactions of the antibody and the antigen enables a target protein to be identified during a complex protein mixture. The first step of western blotting is separating the proteins, using gel electrophoresis. following separation, the proteins are removed from the gel into the membrane, that was treated with blot stain, and gelatin. The gelatin is being used to prevent any nonspecific reactions from occurring. The membrane is then transferred onto the color development solution, which contains the antibody. The antibody is linked to a reporter enzyme that produce color, purple in this case, which allows the antibody to be easily detected. The purpose of this experiment is to identify albumin, transferrin and gamma globulins in serum and the distance that separate the two species on the evolutionary tree based on the intensity of the purple