In vitro, in vivo and in silico drug-drug interaction study between Vildagliptin and Bisoprolol fumarate. ABSTRACT
Objective: Present study was aimed to evaluate the in vitro, in vivo and in silico complexation nature and strength of complex which may be formed due to interaction between Vildagliptin and Bisoprolol fumarate.
Methods: The interaction of Vildagliptin and Bisoprolol fumarate has been studied in aqueous systems at a fixed temperature (370C) at pH 1.0, 3.0 and 6.8 by using some physical methods as spectral observation, Job’s method of continuous variation, Ardon’s method. In silico complexation was measured by NMR prediction and DDI-CPI analysis.
Results: From spectrophotometric study, Vildagliptin and Bisoprolol fumarate give different …show more content…
The jobs plot was obtained by plotting absorbance difference against the mole fraction of the each drug at pH 1, 3, and 6.8. Vildagliptin moderate for 1:1 mixture with Bisoprolol fumarate and slightly lower spectra indicate the formation of 1:1 mixture of Vildagliptin with Bisoprolol fumarate. These may indicate moderate kinetics of complexation between Vildagliptin with Bisoprolol fumarate. The value of stability constant for the drug-drug system at pH 1.0, 3.0 and 6.8 are 1.875 0.7778 and 1.2000 respectively. At pH 1.0 it is found that Vildagliptin form relatively stable complex with Bisoprolol fumarate (stability constant 1.875) is high in comparison to pH 3. From the IR report it
Objective: Present study was aimed to evaluate the in vitro, in vivo and in silico complexation nature and strength of complex which may be formed due to interaction between Vildagliptin and Bisoprolol fumarate.
Methods: The interaction of Vildagliptin and Bisoprolol fumarate has been studied in aqueous systems at a fixed temperature (370C) at pH 1.0, 3.0 and 6.8 by using some physical methods as spectral observation, Job’s method of continuous variation, Ardon’s method. In silico complexation was measured by NMR prediction and DDI-CPI analysis.
Results: From spectrophotometric study, Vildagliptin and Bisoprolol fumarate give different …show more content…
The jobs plot was obtained by plotting absorbance difference against the mole fraction of the each drug at pH 1, 3, and 6.8. Vildagliptin moderate for 1:1 mixture with Bisoprolol fumarate and slightly lower spectra indicate the formation of 1:1 mixture of Vildagliptin with Bisoprolol fumarate. These may indicate moderate kinetics of complexation between Vildagliptin with Bisoprolol fumarate. The value of stability constant for the drug-drug system at pH 1.0, 3.0 and 6.8 are 1.875 0.7778 and 1.2000 respectively. At pH 1.0 it is found that Vildagliptin form relatively stable complex with Bisoprolol fumarate (stability constant 1.875) is high in comparison to pH 3. From the IR report it