Coliscan easygel nutrient broth was stored in the freezer. Around 24 hours prior to sampling, the broth was moved to the refrigerator to thaw. The sampling procedure follows the procedure stated in the Coliscan Easygel Instructions. When we were ready to begin sampling, the thawed broth was placed into a cooler. We started sampling at Cornerstone pond then move to the Wyoming site, then downtown Grand Rapids, then Plainfield, and then Versluis lake. The procedure for Cornerstone pond and Versluis Lake are as follows: first, wearing waders, we walk about 3 feet into the water; then we open a sterile pipette and label a vial of nutrient broth; we use a pipette to deliver 3mL of water into the vial; then, we recap the vial and return it to the
