Vanillin Experiment

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1.0 Introduction
4-hydroxy-3methbenzaldehyde is the scientific name for Vanillin. What is vanillin? It is a substance that adds flavor to the special taste and smell in food. It is extracted from high quality beans (1). A specific solvent named ethanol can be used to extract vanillin. There are two types of vanillin namely natural vanillin and artificial vanillin. There is artificial vanillin in the market because natural vanillin is costly. Before a chemical experiment or process is carried out, the concentration of an unknown solution will always be determined. After this step is done, a calibration curve will be drawn. The unknown concentration of the solution can be determined from the linear equation of the calibration curve where Beer’s
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This can be done by shaking the separating funnel gently and inverting it a few times for approximately 2 minutes. After this step is complete, the stopper was opened. This is to release excess vapour pressure. The contents are then separated. After the layers have settled and two layers are visible, the separating funnel was hung on the retort stand. Next, the organic solution located at the bottom was drained off. The procedures mentioned were repeated using another 20ml of portion of dichloromethane and acquired organic extracts, mostly containing vanillin were combined. Then, poured into another clean separating funnel. Next, 40 ml of 0.1M of sodium hydroxide, NaOH solution was measured and poured into a clean 100 ml beaker. The sodium hydroxide solution was then added into the separating funnel that has the organic extract from the earlier procedure. The separating funnel is then shook gently for 2 minutes in order to mix the contents together. Then, the aqueous extract solution was poured into a 250 ml volumetric flask. Next, make up to the volume with 0.1 M sodium hydroxide …show more content…
The cuvettes were held on the top and a piece of clean tissue was used to wipe the body of the cuvettes. This is to ensure that there are no impurities present on the cuvette. For the inner section of the cuvette, the cuvettes were rinsed twice with distilled water to ensure its cleanliness. Next, a cuvette was then rinsed twice using Standard solution 1. This procedure is crucial as it ensures that there are no foreign compound in the cuvette other than the standard solution itself. Lastly, the cuvette containing standard solution 1 was then placed into the cuvette storage box. The procedures were then repeated using Standard solutions 2,3,4,5 and also the sample solution that was prepared in part

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