In this research, mesoporous material KIT-6 silica with high surface area (924.5 m2/g) and pore diameter (9.71 nm) was successfully synthesized using rice husk ash as a silica source. According to the SAXS analysis and TEM micrographs, the silicate structure of as-prepared KIT-was not distrupted by the functionalization and activation treatments with 3-APTES and glutaraldehyde, but showed a significant decrease in pore diameter and total pore volume as the compound was covalently bonded to the KIT-6 surface. Besides, the pore diameter of KIT-6 obtained was considered sufficient to accommodate the medium size protein molecule. The FESEM image gave supporting evidence for the mesoporous silica KIT-6 with a gyroid structure in complement to the SAXS analysis, signifying the KIT-6 with Ia3d symmetry and the 3D-interconnecting pore network. Besides that, the FTIR spectra confirmed the structure of KIT-6 and incorporation of 3-APTES and glutaraldehyde into the KIT-6. According to these important findings, the KIT-6 could be a potential support for CRL immobilization.
Then, the incorporation of CRL into the KIT-6 was also characterized using SAXS and TEM analysis that showed no significant alteration in the KIT-6 structure before and after CRL immobilization. However, the surface area and pore volume was decreased, respectively, with the incorporation of KIT-6 (924.5 m2/g; 9.71 nm), after functionalization and derivatization of KIT-6/a/g (356.7 m2/g; 5.95 nm), immobilization of
Then, the incorporation of CRL into the KIT-6 was also characterized using SAXS and TEM analysis that showed no significant alteration in the KIT-6 structure before and after CRL immobilization. However, the surface area and pore volume was decreased, respectively, with the incorporation of KIT-6 (924.5 m2/g; 9.71 nm), after functionalization and derivatization of KIT-6/a/g (356.7 m2/g; 5.95 nm), immobilization of