Using Conditional Knockout Mouse Technology Essays

1028 Words Nov 10th, 2016 5 Pages
Knockout mouse technology is valuable for determining the purposes and operations of genes that have not been sufficiently studied in the laboratory. These genes are replaced with mutant or diseased versions of themselves, permitting researchers to discover and discern how losing the normal function of certain genes can affect an organism. Through the use of this technology, the mouse genome has been extensively mapped and attributed to a variety of functions for specific genes. In this analysis, I will discuss knockout mouse technology, describe the systematic process used in the lab, and introduce the concept of conditional knockout mouse technology pertaining to Cre (bacteria) recombination. In order to begin the process of manipulating a gene in the mouse, it is important to be able to identify that particular gene. This gene is represented by a normal exon, which can be identified via its codon and amino acid sequences. Knowing the genetic properties of the gene permits the synthetic creation of a mutant version of the gene in the lab, which is what will be used to replace the gene being studied. Knockout mouse technology involves several other components, as well, which will be introduced now. Hypothetically, assume that a researcher has a white, pregnant female mouse that has a gene with an unknown function. In order to mutate this gene, the researcher uses a culture growth of embryonic stem cells from a different color mouse (agouti color) and induces the specific…

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