Unknown Microbiology Lab Report Essay examples

1131 Words Dec 13th, 2011 5 Pages
UNKNOW BACTERIA LAB REPORT
UNKNOWN 36

Introduction

The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others make antibiotics used today. This lab was completed by using the methods learned thus far in identification of bacteria.

Materials and Methods

A mixed culture of two unknown bacteria was provided by the instructor. The methods used for
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Single streak at line of stab | Sulfide negative Indole negativeNon motile |

Table 2: Biochemical Test Results (Bacteria # 2) TEST | PURPOSE | REAGENTS | OBSERVATIONS | RESULTS | Gram Stain | To determine the Gram reaction of Bacteria # 2 | Crystal violet, Iodine, Alcohol, Safranin | Pink rods | Gram negative rods | EMB Agar plate | To determine the ability to ferment lactose | None | Dark purple streak | Fermentation of Lactose | Enterotube II | Identify bacteria | Kovac’s | Interpreted results using the interpretation color chart. Added reagent resulted in Indole negative | Escherichia coliBiocode 34760 | FLOW CHART 1
UNKNOWN 36
BACTERIA # 1

FLOW CHART 2 UNKNOWN 36 BACTERIA # 2

It has been concluded that the Unknown 36/Bacteria # 1 is Staphylococcus aureus and Unknown 36/Bacteria # 2 is Escherichia coli. This was accomplished by first streaking a BHI/TSA infused plate with the Unknown 36 broth to isolate the two bacteria. Once two different bacterial growths were observed, each different bacterium was streaked on individual TSA plates to isolate colonies for Gram staining and biochemical tests. Gram staining resulted in Bacteria # 1 being Gram positive cocci and Bacteria # 2 being Gram

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