Two temperatures of turnip extract were tested: 0°C and 100°C. Three trials were run at each temperature to obtain an average and lessen the effect of error in individual trials.
For the 0°C trials, turnip extract with an unknown concentration of turnip peroxidase was obtained and kept on ice for the duration. A control mixture was prepared in a test tube which contained 1.0 mL turnip extract, 8.9 mL buffer pH 6.8, and 0.1 mL guaiacol . A Spectronic 20 spectrophotometer was set to 460 nm and blanked using the control. Then two test tubes were prepared with the following mixtures:
Tube 1
Tube 2
Buffer pH 6.8
4.7 mL
4.0 mL
Turnip extract
1.0 mL
Guaiacol
0.1 mL
1.0% H2O2
0.2 mL
Turnip dilution:
1:4
The two tubes’ contents were mixed
For the 0°C trials, turnip extract with an unknown concentration of turnip peroxidase was obtained and kept on ice for the duration. A control mixture was prepared in a test tube which contained 1.0 mL turnip extract, 8.9 mL buffer pH 6.8, and 0.1 mL guaiacol . A Spectronic 20 spectrophotometer was set to 460 nm and blanked using the control. Then two test tubes were prepared with the following mixtures:
Tube 1
Tube 2
Buffer pH 6.8
4.7 mL
4.0 mL
Turnip extract
1.0 mL
Guaiacol
0.1 mL
1.0% H2O2
0.2 mL
Turnip dilution:
1:4
The two tubes’ contents were mixed