Plant Material Collection Fully grown whole plants of Tribulus terrestris Linn. were collected from different localities of Chennai, Tamilnadu. The fruits were separated out from the plants and washed in running tap water to remove adhering dirt and soil particles and dried first under shade in room temperature and secondly in a hot-air oven for complete drying at 50-60˚C. The dried fruits were made into coarse powder by grinding and stored in labelled bottles for further study and extracted with organic solvents like Ethanol. Fresh thorns also grinded and extracted with same extract.
Preparation of aqueous, methanolic and ethanolic extracts of fresh and dried fruits of Tribulus terrestris Linn.
The fresh fruits of Tribulus terrestris Linn. were weighed out and crushed directly by …show more content…
After the cell reaches the confluence, the various concentrations of the samples were added and incubated for 24 hours. After incubation, the sample was removed from the well and washed with phosphate-buffered saline (pH 7.4) or MEM without serum. 100µl/well (5mg/ml) of 0.5% 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl--tetrazolium bromide (MTT) was added and incubated for 4 hours. After incubation, 1ml of DMSO was added in all the wells .The absorbance at 570nm was measured with UV- Spectrophotometer using DMSO as the blank. Measurements were performed and the concentration required for a 50% inhibition (IC50) was determined graphically. The % cell viability was calculated using the following formula:
% cell viability = A570 of treated cells / A570 of control cells × 100
Graphs are plotted using the % of Cell Viability at Y-axis and concentration of the sample in X-axis. Cell control and sample control is included in each assay to compare the full cell viability in Cytotoxicity and anti-cancer activity
Preparation of aqueous, methanolic and ethanolic extracts of fresh and dried fruits of Tribulus terrestris Linn.
The fresh fruits of Tribulus terrestris Linn. were weighed out and crushed directly by …show more content…
After the cell reaches the confluence, the various concentrations of the samples were added and incubated for 24 hours. After incubation, the sample was removed from the well and washed with phosphate-buffered saline (pH 7.4) or MEM without serum. 100µl/well (5mg/ml) of 0.5% 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl--tetrazolium bromide (MTT) was added and incubated for 4 hours. After incubation, 1ml of DMSO was added in all the wells .The absorbance at 570nm was measured with UV- Spectrophotometer using DMSO as the blank. Measurements were performed and the concentration required for a 50% inhibition (IC50) was determined graphically. The % cell viability was calculated using the following formula:
% cell viability = A570 of treated cells / A570 of control cells × 100
Graphs are plotted using the % of Cell Viability at Y-axis and concentration of the sample in X-axis. Cell control and sample control is included in each assay to compare the full cell viability in Cytotoxicity and anti-cancer activity