Toxicity Testing and Determining the LC50
Introduction
This report discusses an experiment of Toxicity test done in Richland lab to determine the LC50, a test that is used to find out what concentration of a substance in this case household chemicals that will kill 5o percent of the population of the brine shrimps, with the exposure household chemicals such as , Ammonia, Bleach, and Vinegar, and the morality with each solutions. I and my group splitte the Brine shrimps into 4 containers, first one for control group ( no solution will be added to that container), the second one for Ammonia, third one for Bleach and the last one for Vinegar. our primary goal was to calculate the concentration of the Brine shrimps, using the formula % concentration=does/total solution, we calculated the concentration percent for the dose amount of 0, .5, 1, 2, 4 , 8 and 10 Ml in each container. our secondary goal was to add the substance the amounts in ml I mentioned above respectively from small to high amount. My prediction ranking was Ammonia with higher toxic, Bleach with intermediate toxicity, and Vinegar least Toxic. …show more content…
And labeled using Grease pencils
Brine shrimp, Brine solution
Vinegar, Ammonia, and Bleach. about 50 ml of each.
3 pipette pumps
Magnifiers or hand lens
Extra large Beaker for west and another one for tested Brine shrimp
After having all the materials listed we obtain some shrimps in a 25 ml cylinder, add brine to bring the level to a total of 20 ml. We observe the brine shrimp for 5 min without adding a substance and after adding .5 , 1, 2, 4, 8, 10ml of the substance. Wait and observe their reaction after each amount of substance and record the results.
Toxicity Testing: Brine Shrimp Raw Data
NaCl
Brine
ml
Amount
Introduction
This report discusses an experiment of Toxicity test done in Richland lab to determine the LC50, a test that is used to find out what concentration of a substance in this case household chemicals that will kill 5o percent of the population of the brine shrimps, with the exposure household chemicals such as , Ammonia, Bleach, and Vinegar, and the morality with each solutions. I and my group splitte the Brine shrimps into 4 containers, first one for control group ( no solution will be added to that container), the second one for Ammonia, third one for Bleach and the last one for Vinegar. our primary goal was to calculate the concentration of the Brine shrimps, using the formula % concentration=does/total solution, we calculated the concentration percent for the dose amount of 0, .5, 1, 2, 4 , 8 and 10 Ml in each container. our secondary goal was to add the substance the amounts in ml I mentioned above respectively from small to high amount. My prediction ranking was Ammonia with higher toxic, Bleach with intermediate toxicity, and Vinegar least Toxic. …show more content…
And labeled using Grease pencils
Brine shrimp, Brine solution
Vinegar, Ammonia, and Bleach. about 50 ml of each.
3 pipette pumps
Magnifiers or hand lens
Extra large Beaker for west and another one for tested Brine shrimp
After having all the materials listed we obtain some shrimps in a 25 ml cylinder, add brine to bring the level to a total of 20 ml. We observe the brine shrimp for 5 min without adding a substance and after adding .5 , 1, 2, 4, 8, 10ml of the substance. Wait and observe their reaction after each amount of substance and record the results.
Toxicity Testing: Brine Shrimp Raw Data
NaCl
Brine
ml
Amount