In the essence of lawfully securing the ability to invade and search someone else’s property to look for evidence of a crime, two approaches have been examined. One being the totality of circumstance approach which states that all facts and circumstances in the given situation should be looked at in each case individually, meaning that the whole picture needs to be examined in order to determine if there is probable cause to enter someone’s property for searches and seizures; the other approach being the Aguilar-Spinelli two pronged test, which was developed in Aguilar v. Texas and Spinelli v. United States and the two requirements were that, “The magistrate must be informed of the reasons to support the conclusion that such an informant is…
By having a thicker gel, smaller segments can move better and not be cleared out by bigger segments. Polymerase Chain Reaction (PCR) is used to replicate DNA. In Lab 5, we created PCR amplicons by collecting our own DNA through our cheek cells, adding Taq DNA polymerase, dNTPs, primer, and a proper buffer solution into a PCR tube.…
The purpose of this lab is to examine cross sections from the leaves of C3 and C4 plants and to determine the morphological differences between them while relating those differences to their metabolism. In C3 plants the carbon dioxide is first incorporated into a 3-carbon compound. Their stomata are open during the day and photosynthesis takes place throughout the mesophyll cells. In comparison C4 plants, the CO2 is first incorporated into a 4-carbon compound. Their stomata are open during the day and photosynthesis takes place within the inner cells.…
According to http://www.encyclopedia.com, DNA which stands for deoxyribonucleic acid is used for human genetic makeup. It has different sequences of bases and exist in human body. The sequence of it nucleotides are A, T, G, C; or, adenine, thymine, guanine, and cytosine, respectively. A DNA fingerprinting, is a DNA pattern that has a unique sequence such that it can be distinguished from the DNA patterns of other individual.…
Biology 15 Lab # 4 Professor Passerini September 23, 2015 Scot Albert Lab #4 Questions 1a,b,c, 2, 3a,b,c, 4, 5a,b,c, 7, 11c, d, e, 12a,b ---------------------------------------------------------- 1- a-They are found primarily in the thylakoid membranes. b-No. Cyanobacteria do not have distinct nuclei.…
Several tests were accomplished technically and carefully to identify the unknown # 230 to be M.luteus bacteria. Most of the observed results do match the expected ones, except two tests. The observed results for glucose fermentation and oxidase test did not match the expected results. The expected result for fermentation of glucose test was negative while the observed result was positive.…
R.C. 5.9 Mathematics (Mobile Infant) Activity: “Stack Them Up” Materials: Stacking blocks, nesting cups, measuring cups Infants are continually growing in all areas of development. In this activity, the goal is to get the infant to stack their blocks or cups. The teacher would have the child sit on the floor or at a table with their toys.…
Information about the Bacteria Pseudomonas aeruginosa During microbiology lab, an unknown bacteria culture in liquid broth was assigned to be identified by conducting a series of various tests. Nearly twenty different tests were conducted on the bacteria, but the most important of these was Gram staining test, gelatin stab test, and oxidase test. The results of these three tests allowed for the determination of the bacteria genus and species.…
The agarose gel is poured onto a plastic plat forming wells, and then DNA samples are placed in small wells. When samples are added to their relative wells, gel and plastic plate are…
YR 10 Science Evolutionary Theory Alicia Horsfield Evolution can be defined as a gradual process in which a species changes into a different and more complex form. This happens over several generations. Evolution also proves that all species are related through genetic variation. (yourgenome.org, n.e). Charles Darwin (1809-1882) was an English Naturalist, famous for creating the theory of ‘evolution’.…
The number of base pairs for the PGEX-KG (original) plasmid and PGEX-KG SAW1 (clone gene) is different. PGEX-KG- Saw1 has 794 bp more than original plasmid. The single digestion for both PGEX-KG and PGEX-KG-Saw1 will make a single cut in their respective restriction sites. The clone and original plasmids will become linearized and the PGEX-KG-Saw1 will be longer than PGEX-KG because of the insertion of Saw1.…
When finding out unknown bacteria you have to go through several steps to figure out what it could be. I was given unknown bacteria number 28 and asked to run different testing to identify exactly what this bacterium is. I ran 6 test to determine my unknown. These test includes: Gram Stain, SIM, Sucrose, Citrate, MAC, and EMB. Each one of these test are different and they will give a positive or negative feedback which is how we will know which bacterium I have.…
In this experiment, a SDS-PAGE gel was used to analyze the protein samples from the MBP-AP and WT-AP experiments. The samples are then referenced to the ladder to determine the molecular weight of the MBP-AP and WT-AP proteins. Then the UV absorbance of the two proteins from 240 nm to 340 nm is determined using a nanovolume cuvette. The absorbance at 280 nm was then used in conjunction with data from previous experiments to determine the concentration of the MBP-AP and WT-AP protein samples. Results of experiments showed that the SDS-PAGE gel yielded expect bands and the approximate molecular weight of wild type alkaline phosphatase and maltose binding protein-alkaline phosphatase is 49 kDa and 95 kDa, respectively.…
Cheyenne Wilson 5.05 LAB REPORT CANDY CHROMATOGRAPHY BACKGROUND INFORMATION: Paper chromatography is a widely used method of separation. The lab will show the basic techniques of paper chromatography. In this lab, the separation of the dyes used in two different kinds of candy is performed.…
Introduction In this lab report I use two different techniques to identify Unknown A and Unknown B bacteria’s. These techniques are gram staining and metabolic testing. I first used Gram staining to distinguished and identify the bacteria’s. Han Christian discovered gram staining in 1882, he had biopsy a patient lung that had pneumonia.…