Essay about Tlc Analysis of Analgesic Drugs

625 Words Apr 29th, 2013 3 Pages
Name: Bill Marano
Due Date: 4-1-13
Experiment Title: TLC Analysis of Analgesic Drugs
Purpose: The purpose of this experiment is to identify the brand name of an unknown commercial analgesic product by TLC analysis. Unknown #1 solution was run against standard solutions of individual analgesics. The components of the unknown product were identified by comparing characteristic traits, such as Rf, fluorescence and iodine staining, to those exhibited by the standards. The name of the commercial product was then determined using the reference table, Analgesics & Caffeine in Some Common Preparations.
Reactions:

Procedure: Refer to Pavia, et. al., Introduction to Organic Laboratory Techniques; 4th ed., Thomson Learning, pp. 82-86
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1. From Plate #1
2. From Plate #2
Calculations:
Example:
Rf= Distance spot travels/ Distance solvent front travels
Rf of Acetaminophen= 48cm/ 150cm
Rf of Acetaminophen= 0.320

Results:
Table 2: Rf and Identification of TLC Spot Substance | Rf(s) | Spot ID | Acetaminophen | 0.320 | N/A | Aspirin | 0.573 | N/A | Caffeine | 0.093 | N/A | Ibuprofen | 0.622 | N/A | Salicylamide | 0.547 | N/A | Reference Mix: | | | Spot 1^1/1^2 | 0.094 Avg. | Caffeine | Spot 2^1/2^2 | 0.302 Avg. | Acetaminophen | Spot 3^1/3^2 | 0.517 Avg. | Salicylamide | Spot 4^1/4^2 | 0.601 Avg. | Ibuprofen | Unknown #1: | | | Spot 1 | 0.101 | Caffeine | Spot 2 | 0.574 | Aspirin |

Unknown #1 Commercial Product Name: Anacin or Cope (Both contain Aspirin and Caffeine)
Conclusion:
The Identity of unknown #1 is either Anacin or Cope because the spot locations on the plates when compared to the known analgesics as well as the reference samples indicate the presence of aspirin and caffeine. Also, the Rf values of the unknown spots indicate the presence of aspirin and caffeine when compared to the Rf of

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