OBJECTIVE For the first week of the lab, the objectives were to isolate and test for the presence of major components of milk, including proteins, carbohydrates, fats, vitamins, and minerals and to determine the presence of the various components of milk in the various fractions using qualitative tests for these substances. For the second week of the lab, the there were three objectives that related to the procedure: to determine the calcium concentration of milk using the technique of titration, to us the concepts of molarity and stoichiometry to perform a quantitative analysis of metal ions found in samples, and to understand and work with other concentration units like ppm.
INTRODUCTION Within the lab experiment, qualitative tests were conducted to determine the presence of the major components of milk, i.e., proteins, carbohydrates, fats, vitamins, and minerals. Furthermore, the milk calcium content of the milk was concluded using the technique of …show more content…
Exactly 5.00 mL of milk was pipetted into a clean 250 mL Erlenmeyer flask and 100 mL of distilled water was added, as well as 10 mL of buffer solution, 1 mL MgEDTA, and 5-6 drops of Eriochrome Black T indicator. The initial volume of the EDTA in the burette was recorded and then the titration started and continued until the sample solution changed colors to pure blue, all the while the solution was stirred. Near the endpoint, the EDTA solution was added dropwise to ensure that the correct final volume was indicated. Once reached, the final volume of the EDTA solution in the burette was recorded and used to determine the change in volume, the molarity of the calcium content, and the concentration of the calcium content in ppm, all to three significant figures. The procedure was repeated to conclude two values that were within 0.10 mL of one
INTRODUCTION Within the lab experiment, qualitative tests were conducted to determine the presence of the major components of milk, i.e., proteins, carbohydrates, fats, vitamins, and minerals. Furthermore, the milk calcium content of the milk was concluded using the technique of …show more content…
Exactly 5.00 mL of milk was pipetted into a clean 250 mL Erlenmeyer flask and 100 mL of distilled water was added, as well as 10 mL of buffer solution, 1 mL MgEDTA, and 5-6 drops of Eriochrome Black T indicator. The initial volume of the EDTA in the burette was recorded and then the titration started and continued until the sample solution changed colors to pure blue, all the while the solution was stirred. Near the endpoint, the EDTA solution was added dropwise to ensure that the correct final volume was indicated. Once reached, the final volume of the EDTA solution in the burette was recorded and used to determine the change in volume, the molarity of the calcium content, and the concentration of the calcium content in ppm, all to three significant figures. The procedure was repeated to conclude two values that were within 0.10 mL of one