Tinidazole Essay

2519 Words 11 Pages
Materials and Methods
4.1 Materials and equipments Table no. 4.1: Materials/Chemicals used in the project work
S.No. Material/Chemicals Property Source
1. Tinidazole Pure drug Trozan Pharma
2. Guar gum Polymer Central drug house Pvt. Ltd.
New Delhi
3. Starch Disintegrant Nice Chemicals
4. Micro crystalline cellulose Diluent, Disintegrant Loba Chemie, Mumbai,India
5. Talc Lubricant Nice Chemicals Pvt. Ltd.
6. Acetone Solvent Nice Chemicals Pvt. Ltd.
7. Potassium dihydrogen phosphate Buffering agent Loba Chemie, Mumbai, India
8. Sodium hydroxide pellets Buffering agent Nice Chemicals Pvt. Ltd.
9. Eudragit S 100 Coating polymer PC Chem, Mumbai, India
10. Castor oil Plasticizer Nice Chemicals Pvt. Ltd.

Table No. 4.2: Equipments used in
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4.2.1.2.2 IR spectroscopy IR spectra of Tinidazole A small amount of Tinidazole was taken on butter paper then it was placed in desiccator for removal of moisture from the same. After the removal of moisture the sample was placed under IR lamp for complete removal of moisture from the sample. Completely dried sample was placed on IR crystals to obtain the IR spectra of sample.
4.2.1.3 Solubility Solubility of Tinidazole Accurately weight 100 mg of Tinidazole was dissolved in required quantities of distilled water and methanol
4.2.2 Preformulation studies of carrier system
4.2.2.1 Organoleptic properties Appearance: A small quantity of guar gum was separately evaluated visually on a fresh butter paper.
Colour: A small quantity of guar gum was separately evaluated visually on a fresh butter paper.
Odour: A small quantity of guar gum was taken and smelled separately to get odour.
Taste: Very small quantity was taken in mouth to get the taste of guar gum.
4.2.2.2 Identification of carrier system
4.2.2.2.1 IR
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Serial dilutions were carried out to get different concentration 1, 2, 3, 4, 5, 6, 7, 8, 9, 10μg/ml. The absorbance was measured at λmax on double beam UV- VIS spectrophotometer (UV-1800) against blank.
4.2.5.2 Preparation of calibration curve of Tinidazole in phosphate buffer of pH 7.4
The stock solution was further diluted with phosphate buffer of pH 7.4. Serial dilutions were carried out to get different concentration 1, 2, 3, 4, 5, 6, 7, 8, 9, 10μg/ml. The absorbance was measured at λmax on double beam UV- VIS spectrophotometer (UV-1800) against blank.
4.2.5.3 Preparation of calibration curve of Tinidazole in phosphate buffer of pH 6.8 The stock solution was further diluted with phosphate buffer of pH 6.8. Serial dilutions were carried out to get different concentration 1, 2, 3, 4, 5, 6, 7, 8, 9, 10μg/ml. The absorbance was measured at λmax on double beam UV- VIS spectrophotometer (UV-1800) against blank.
4.2.6 Formation of Tinidazole guar gum solid

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