Data: Data Table 1: Determining Percent Yield of the Product Calculations: Moles of reactant…
The physiochemical properties of this combination tablets were assessed through the evaluation of uniformity of tablet weight, thickness test, hardness test, friability test,disintegration test, dissolution test and potency test according to the standard method. They have generally two type of test. First compendial test and another one in non compendial test. Compendial tests are test methods that are described in the pharmacopoeias like United States Pharmacopeia (USP), British Pharmacopoeia (BP) etc. They are also known as official tests.…
Task 3 P1 When using thin layer chromatography (TLC) to test for purity of aspirin it is a quick and simple method. Firstly when using the TLC method you need to have a TLC sheet and mark on the sheet 0.5cm up on the sheet and draw a line, make sure you don’t press too hard on the paper as this will disrupt the absorbent on the paper. You need to make sure the samples have been marked on the paper so you don’t get mixed up and leave a gap between the aspirin samples so they don’t run together as this will give an inaccurate result. Next get a 1% concentrated sample of a known purity of salicylic acid as this is suitable for the TLC analysis, then obtain a small glass rod (micro capillary) and use this to dip in the solution and on to the paper in the position where the sample of aspirin is named.…
Thin-Layer Chromatography Lab 11/12/14 Beginning Question To what extent does the polarity of a substance affect the distance it travels during TLC using water as the solvent? Safety Concerns The substances used during the TLC process could possibly be dangerous, so it would be best to avoid getting them on the hands.…
According to the average density and the boiling point of the experiment, the probable identify liquid of M4C is Propanol (Isopropyl alcohol), with the density value is (0.788 g/mL) and boiling point is (82.67 *C) compare with the literature value of density (0.785 g/mL) and the boiling point (82.4 *C), which Propanol (Isopropyl alcohol) should be the liquid of M4C. The Propanol (Isopropyl alcohol) has no color. The key of identify the unknown is the boiling point and the density, because if depend on the density, we might have multiple liquid and it not very accurate, but since we can determine the boiling point, the number of boiling point and density will help us to find out the most precise unknown liquid. The most precise is the pipette,…
Methods Column chromatography was set up using a 60 ml syringe clamped to a ring stand. The syringe was plugged using a small amount of cotton. An Erlenmeyer flask was placed underneath the column to collect any excess material. Eleven 20 ml vials were labeled numbers 1 through 11. Silica gel was measured to 42 ml using a graduated cylinder and then moved into a 150 ml beaker.…
High Performance Liquid Chromatography Lab Abstract High performance liquid chromatography or HPLC is the process in which mixtures are physically separated according to their polarity. The lab conducted, the separation of dyes in grape Kool-Aid, demonstrated the concept. This was done to display how different alcohols and their concentrations have different polarities, in which ties into HPLC. The lab consists of testing Kool-Aid, water, and different concentrations of methanol and isopropanol; 5%, 20%, and 60%. To start, one must pour 10 ml of the Kool-Aid into a syringe that was connected to a C-18 absorbent.…
This procedure is a fast way to compare the purity of a sample through the retention factor (Rf ) values, which is the distance the caffeine and other organic compounds have traveled divided by the…
The objective of the experiment was to separate chlorophyll a and chlorophyll b and the alpha and beta conformations using the paper and column chromatography. The two techniques that were used to separate and identify the plant pigments were the paper chromatography and column chromatography. For the mixture to be separated, it must first be applied to a stationary phase. The stationary phase in the paper chromatography experiment was the filter paper. The mobile phase used in this experiment to help separate the pigments were the hexane and acetone solution.…
Thus, the order of boiling points between the two compounds is: 1-pentanol >…
TLC and HPTLC Introductions Thin layer chromatography (TLC) is derived from liquid chromatography where liquid serves as mobile phase and thin layer of substance on a flat late surface serves as stationary phase. The thin layer of materials act as sorbent while the mobile phase known as solvent. Solvent will send the solutes through the sorbent depends on the strength of the mobile phase as it dissolved the solute and send them up the plate against gravity ,and resistance of sorbent when it attracts the solute out from the solvent[4]. The molecule in the solute moves in a stop-and-go motion as they are repeatedly absorbed and desorbed along the plate.…