Specifically group three was given 25mg/ 4mL of thiamine hydrochloride. Group four was given the same amount of zinc. Both thiamine hydrochloride and zinc were given to the last group. Gastric gavage was the procedure used to give the components. Environment and food for all subjects were consistent. After six week five rats from each group were put in a metabolic chamber and their urine was collected. After collection the rats were decapitated and their blood, liver, kidney, and brain tissues were harvested. The remaining twenty rats from group two were split into four groups of five and given identical exposure of groups one, three, four, and five for six days. The remaining rats were put through the same process as the rats the week prior. Urine specimen and tissue samples from the blood, liver, kidney, and brain were collected once again. The specimens and tissue samples were then used in analysis. To start, delta-aminolevulinic acid dehydratase activity was determined by the Berlin and Schaller method. This process consisted of first hemolyzing a 0.2 heparinized blood sample and 1.3 mL of water by incubating it at 37oC. From there, 10 mL of the solution was extracted and incubated again for one hour …show more content…
From there, lead was isolated from MIBK. Last but not least, Zinc protoporphyrin levels were determined by viewing a single drop of blood on haematofluometer. Wet tissue digestion and spectroscopy were utilized to estimate the zinc concentration of tissues. Measurements were taken at 213.8 nm. There were several significant results of this study. One was the delta-aminolevulinic acid dehydratase (delta-ALA) activity in the rats who had been supplemented with anything vitamin or mineral in the lead exposure phase. The mean delta-ALA activity for rats who were gave thiamine, zinc, or both was increased (P<0.001) after lead exposure. The author observed that rats supplemented before or after with thiamine or more importantly zinc had an increased delta-ALA value and decrease lead levels. Values similar to the values seen through supplementation during lead exposure have been in prior experiments. Taking this into account along with the results of the experiment, the author believe that the hypothesis was supported. The strengths of this study were the identical dietary and nutritional parameters. One of the weakness of this experiment was