Cancers are managed through effective nutrition to supplement drugs, and it is generally believed that good nutrition is effective in preventing and minimizing cancer. Indeed, many plant products including complex carbohydrates have been proven to have bio-functional role, including anti-proliferative, autophagy and apoptosis activity [25,26].
Pectin, especially modified pectins have been found to possess anticancer activity. The most well-known pectin in cancer therapy is modified citrus pectin, which is used in prostate cancer therapy with laudable success, it was found to increase the doubling time of prostate specific antigen in men with prostate cancer [27]. The use of modified pectins in cancer research has mainly focused on acid, enzymatic and heat modified pectins. However, there exist sonication, a physical method of pectin modification which is cheap, fast, easy to upscale and efficient with no post treatment cleaning steps [28]. Hence the current study assessed the anticancer activity of ultrasonic modified sweet potato pectin against colon cancer cell line (HT-29), with a view of evaluating its possible potential as anti-cancer agent against the colon cancer focusing on specific end points.
2. Methodology
2.1. Materials
Cell medium RPMI 1640 (Sigma Aldrich, China), …show more content…
A solution of 0.0025g/ml of sweet potato pectin in deionized water, the mixture was stirred on a magnetic stirrer for 1 hour. Thereafter, the solution was sonicated at either 200W or 400Wltrasonic power levels, and constant frequency 25 kHz, duty cycle 60% and the sonication time was fixed at 20minutes, sonication was done at ice cold temperature. The sonicated pectin solutions were frozen at −45°C and freeze dried lyophilized. The dry material was stored at 4°C. Fresh pectin solution in PBS were made just before being added to the cells for the
Pectin, especially modified pectins have been found to possess anticancer activity. The most well-known pectin in cancer therapy is modified citrus pectin, which is used in prostate cancer therapy with laudable success, it was found to increase the doubling time of prostate specific antigen in men with prostate cancer [27]. The use of modified pectins in cancer research has mainly focused on acid, enzymatic and heat modified pectins. However, there exist sonication, a physical method of pectin modification which is cheap, fast, easy to upscale and efficient with no post treatment cleaning steps [28]. Hence the current study assessed the anticancer activity of ultrasonic modified sweet potato pectin against colon cancer cell line (HT-29), with a view of evaluating its possible potential as anti-cancer agent against the colon cancer focusing on specific end points.
2. Methodology
2.1. Materials
Cell medium RPMI 1640 (Sigma Aldrich, China), …show more content…
A solution of 0.0025g/ml of sweet potato pectin in deionized water, the mixture was stirred on a magnetic stirrer for 1 hour. Thereafter, the solution was sonicated at either 200W or 400Wltrasonic power levels, and constant frequency 25 kHz, duty cycle 60% and the sonication time was fixed at 20minutes, sonication was done at ice cold temperature. The sonicated pectin solutions were frozen at −45°C and freeze dried lyophilized. The dry material was stored at 4°C. Fresh pectin solution in PBS were made just before being added to the cells for the