The Successful Synthesis Of Oligonucleotides Essay

1400 Words Nov 28th, 2016 6 Pages
Oligonucleotides carrying a variety of chemical modifications including conjugates are finding increasing applications in therapeutics, diagnostics, functional genomics, proteomics, and as research tools in chemical and molecular biology. The successful synthesis of oligonucleotides primarily depends on the use of appropriately protected nucleoside building blocks including the exocyclic amino group of the nucleobase, the hydroxyl groups at the 2-, and 5-positions of the sugar units, and the internucleotide phospho-linkage. This unit is a thoroughly revised update of the Unit 2.1 entitled “Nucleobase Protection of Deoxyribo- and Ribonucleosides,” and describes various protecting groups and recent development, allowing reliable oligonucleotide synthesis, that have been reported for the nucleobases since the publication of the Unit 2.1. In addition, protecting groups for the imide/lactam function of thymine/uracil and guanine, respectively, are described in some cases, to prevent irreversible nucleobase modifications that may occur in the presence of alkylating or other reagents used in oligonucleotide synthesis.

DNA and RNA are biopolymers comprised of nucleotide units. The synthesis of DNA and RNA as segments consisting of several nucleotides each, referred to as oligonucleotides, is quite a challenging endeavor, requiring the rapid and quantitative coupling of nucleosidic units to form internucleotidic linkages. In order to…

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