INTRODUCTION
The objective of this experiment was to group a variety of samples of matter into subcategories of different macromolecules, which were determined by the use of various tests, as well as designated negative and positive control samples.
Chemical samples may be experimentally identified as specific macromolecules through treatment with Benedict’s solution, the iodine test, or the Biuret test; each, when positive, identifying reducing sugars, starch or glycogen, and protein, respectively (Cardoso, M. B., Samios, D., da Silveira, N. P., Rodembusch, F. S., & Stefani, V., 2007; Kumar J, 2011). This laboratory used the aforementioned three tests to identify eleven samples of which the nature of the sample was given, alongside a final unknown sample’s chemical compositions.
Positive and negative controls are what stabilize the basis of this experiment. Through comparing all given substances’ results to a predetermined substance that will, under proper conditions, result in a positive test, and one that will result in a negative result for a test, one can ensure the accuracy of the experiment by lowering the possibility of false negative or positive results (Pelt-Verkuil, Belkum, & Hays, 2008). Both types of controls were used for all three tests.
MATERIALS AND METHODS …show more content…
The one percent sucrose solution was deemed the positive control, and thus showed a positive result for the test (Kumar, 2011; Pelt-Verkuil et al., 2008). The positive result for all five of the aforementioned samples is attributed to all of the samples being some type of reducing sugar (Kumar, 2011). The solutions do not contain protein, nor starch or glycogen, due to their testing negative for both the Biuret and iodine test (Cardoso et al., 2007; Kumar,
The objective of this experiment was to group a variety of samples of matter into subcategories of different macromolecules, which were determined by the use of various tests, as well as designated negative and positive control samples.
Chemical samples may be experimentally identified as specific macromolecules through treatment with Benedict’s solution, the iodine test, or the Biuret test; each, when positive, identifying reducing sugars, starch or glycogen, and protein, respectively (Cardoso, M. B., Samios, D., da Silveira, N. P., Rodembusch, F. S., & Stefani, V., 2007; Kumar J, 2011). This laboratory used the aforementioned three tests to identify eleven samples of which the nature of the sample was given, alongside a final unknown sample’s chemical compositions.
Positive and negative controls are what stabilize the basis of this experiment. Through comparing all given substances’ results to a predetermined substance that will, under proper conditions, result in a positive test, and one that will result in a negative result for a test, one can ensure the accuracy of the experiment by lowering the possibility of false negative or positive results (Pelt-Verkuil, Belkum, & Hays, 2008). Both types of controls were used for all three tests.
MATERIALS AND METHODS …show more content…
The one percent sucrose solution was deemed the positive control, and thus showed a positive result for the test (Kumar, 2011; Pelt-Verkuil et al., 2008). The positive result for all five of the aforementioned samples is attributed to all of the samples being some type of reducing sugar (Kumar, 2011). The solutions do not contain protein, nor starch or glycogen, due to their testing negative for both the Biuret and iodine test (Cardoso et al., 2007; Kumar,