1. The background and significance of the project
DNA (deoxyribonucleic acid) is the main chemical components of Chromosome, and is the material composition of genes. In the breeding process, the parent is passed to the offspring to copy part of their own DNA, thus completing the spread of traits.
DNA is well-suited for biological information storage. The DNA backbone is resistant to cleavage, and both strands of the double-stranded structure store the same biological information.
DNA analysis is important in genetic, forensic science and medical research. PCR (Polymerase Chain Reaction) device has become an important analytical equipment of molecular biology. It is an instrument that gene replication of the DNA polymerase in the specified temperature. PCR technology can be widely used in the …show more content…
The temperature control indicators mainly refer to the accuracy and uniformity of temperature, speed of heating and cooling. The accuracy of the temperature refers to the temperature of the sample is consistent with the set temperature, which is directly related to the success of the experiment.
2. Temperature control system for PCR instrument
PCR process is divided into three steps:
1.Denaturation (94-96℃): this breaks the weak hydrogen bonds that hold DNA strands together in a helix, allowing the strands to separate creating single stranded DNA.
2.Annealing(less than 65℃): As the temperature falls, the primer combine to complementary sequence in template DNA
3.Elongation(70-75℃): DNA polymerase extends the primers, adding nucleotides onto the primer in a sequential manner, using the target DNA as a template.
The above three steps for a cycle; the product of each loop can be used as a template for the next cycle. This allows more DNA to be produced from a very small sample by creating large number of copies of the DNA in the
DNA (deoxyribonucleic acid) is the main chemical components of Chromosome, and is the material composition of genes. In the breeding process, the parent is passed to the offspring to copy part of their own DNA, thus completing the spread of traits.
DNA is well-suited for biological information storage. The DNA backbone is resistant to cleavage, and both strands of the double-stranded structure store the same biological information.
DNA analysis is important in genetic, forensic science and medical research. PCR (Polymerase Chain Reaction) device has become an important analytical equipment of molecular biology. It is an instrument that gene replication of the DNA polymerase in the specified temperature. PCR technology can be widely used in the …show more content…
The temperature control indicators mainly refer to the accuracy and uniformity of temperature, speed of heating and cooling. The accuracy of the temperature refers to the temperature of the sample is consistent with the set temperature, which is directly related to the success of the experiment.
2. Temperature control system for PCR instrument
PCR process is divided into three steps:
1.Denaturation (94-96℃): this breaks the weak hydrogen bonds that hold DNA strands together in a helix, allowing the strands to separate creating single stranded DNA.
2.Annealing(less than 65℃): As the temperature falls, the primer combine to complementary sequence in template DNA
3.Elongation(70-75℃): DNA polymerase extends the primers, adding nucleotides onto the primer in a sequential manner, using the target DNA as a template.
The above three steps for a cycle; the product of each loop can be used as a template for the next cycle. This allows more DNA to be produced from a very small sample by creating large number of copies of the DNA in the