Essay on The Importance Of Kinesin Mediated Axonal Transport
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A. Specific Aims. Funded study proposed the following three specific aims.
Aim 1: Elucidate the role of kinesin mediated axonal transport in long-term depression (LTD), a form of long-term synaptic plasticity.
Aim 2: Test the hypothesis that the Wnt-signaling pathway regulates axonal transport during LTD and LTF.
Aim 3: Elucidate the role of acetylation of microtubules in regulating kinesin-mediated transport during LTM.
B. Studies and Results. We are happy to say that we have made substantial progress in the first two aims. Aim 3 will be initiated in Year 3. Below we describe the progress in Aim 1 and 2. Because both Aim 1 and 2 are carried out at the same time, a combined description of the progress is given below.
The long-term goal of this project is to understand the significance of axonal transport to synapses for establishing and maintaining long-term memories at sensory neuron-motor neuron synapses of gill-withdrawal reflex of Aplysia. Below we describe our key findings.
1. Development of an automated assay for quantifying mitochondrial transport in neurons. Manual analysis of mitochondrial transport is slow and labor intensive. To develop and test the feasibility of collecting time-lapse data with a robotic microscope and then using software to analyze motion, hippocampal…