Place one droplet of reagent onto the specimen swab. Observe the reaction of the swab. A positive reaction will turn violet to purple with no delay. A delayed reaction should be …show more content…
Mix together the unknown stock culture into the hydrogen peroxide drop. Observe the absence or presence of bubbling.
KOH (Potassium Hydroxide) Test: Place a drop of 3% KOH on a slide. Use a toothpick and transfer a good amount of the bacteria to the droplet of KOH. Stir it together. Raise and lower the toothpick off the slide to observe the results. Obligately aerobic organisms will grow on top but not in the stab.
Motility Agar: Obtain a motility agar tube. Using an inoculation pick stab 2/3 of the way into the agar from your unknown stock culture as straight as possible. Incubate for up to 72 hours. Then observe your culture by holding it up to a light source. Results depend on visual stance, if the growth can be seen from halfway across the room it may indicate a positive result, if it is not visible from across the room then may indicate the result to be negative. (depending on eyesight)
Thioglycolate Broth For Oxygen Requirements: Label a thiogly collate broth tube then, using a sterile stab inoculate the organism into the tube by moving the stab up and down in a straight manner. Incubate for up to 48 hours then observe the