The Effect of Decreased Amounts of DOPA on the Production of Dopachrome
Intro
Enzymes are a catalyst, typically a protein, that affects the rate of a chemical reaction. They allow these complex reactions to occur at a low temperature with less energy usage. In enzyme-catalyzed reactions, a substrate, the substance to be acted upon, binds to the active site on an enzyme to form the product. The active site is the only part of the enzyme that reacts with the substrate. For the substrate to become chemically changed, the enzyme must form a complex with the substrate first. When the substrate bonds with the enzyme through the active site, that’s when the new product can be made. When the product reaches full development, it is then released from …show more content…
Within our results we found that having the two mL be the control (blue line in Figure 1) the 1.5mL (grey line in Figure 1) and three mL (red line in Figure 1) increase along with it. Although they didn’t increase at a steady rate or start at the same absorbance, they still increased along with the positive control. The three mL cuvette actually started before the positive control at .333nm, while the positive control started at .279nm. This could be for any number of reasons, but the main one being that the increase of substrate to enzyme started off greater than the two mL cuvette. As you can see in Figure 1, the three mL eventually started to slow down, which could mean that the substrate was starting to “run” out of enzymes to keep the reaction rate up. Now the 1.5mL did the same thing but between 50 and 100 seconds the slope had a higher increase, which this could be where the substrate- enzyme complex started to kick in. As you may or may not notice though, the negative control is not in Figure 1. This is because it is a negative control, the numbers were decreasing and if it was added to the graph it would pull all the numbers down and skew it and bring all the numbers down due to the
Intro
Enzymes are a catalyst, typically a protein, that affects the rate of a chemical reaction. They allow these complex reactions to occur at a low temperature with less energy usage. In enzyme-catalyzed reactions, a substrate, the substance to be acted upon, binds to the active site on an enzyme to form the product. The active site is the only part of the enzyme that reacts with the substrate. For the substrate to become chemically changed, the enzyme must form a complex with the substrate first. When the substrate bonds with the enzyme through the active site, that’s when the new product can be made. When the product reaches full development, it is then released from …show more content…
Within our results we found that having the two mL be the control (blue line in Figure 1) the 1.5mL (grey line in Figure 1) and three mL (red line in Figure 1) increase along with it. Although they didn’t increase at a steady rate or start at the same absorbance, they still increased along with the positive control. The three mL cuvette actually started before the positive control at .333nm, while the positive control started at .279nm. This could be for any number of reasons, but the main one being that the increase of substrate to enzyme started off greater than the two mL cuvette. As you can see in Figure 1, the three mL eventually started to slow down, which could mean that the substrate was starting to “run” out of enzymes to keep the reaction rate up. Now the 1.5mL did the same thing but between 50 and 100 seconds the slope had a higher increase, which this could be where the substrate- enzyme complex started to kick in. As you may or may not notice though, the negative control is not in Figure 1. This is because it is a negative control, the numbers were decreasing and if it was added to the graph it would pull all the numbers down and skew it and bring all the numbers down due to the