Plasmid Electroporation Lab Report

Decent Essays
In order to accomplish the goal of genome synthesizing, the scientist needed to use certain equipment that would allow them to visualize and make data for the experiment. Previous work demonstrated that experimentation with a single codon was successful. Confirmation that the 57 codon versions of ads and tyrS were functional in vivo and that recoded and nsAA dependent and maintained fitness and provided extremely low escape rates as previously reported. Electroporation protocol transformed several cell types, at rather high efficiencies without various plasmids. Abstract E.coli can be transformed to extremely high efficiencies by subjecting a mixture of cells and DNA to brief, but the intense electrical field of exponential decay …show more content…
Several steps using these materials allows the plasmid DNA with DNA and heat shocking the cells then placing in an ice bath then incubating the solution then placing the solution on the E.coli plates and allowing them to incubate overnight will show if the new codons were effective (Edvotek, 2013).
The estimated cost of this experiment for reconstructing rE.coli-57 is 1 million dollars. This experiment has opened new doors for the future of biotechnology.
The article was presented in a thoroughly scientific manner in which they addressed their purpose, methods, and findings. Though it took a few reads to fully understand the material, it provided a clear and useful explanation. Their thesis offers potential health advances but also comes with a potential danger. Considering the authors’ thesis, they successfully provided a framework to develop a synthetic organism through the process of rewriting genomes. After trial and errors, the authors explained the information into graphs. The 12 graphs are useful material that other researchers can use to build on the existing

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