The Bile Esculin test was performed on a Bile Esculin Agar slant by inoculating the slant in a zig-zag pattern and was stabbed using an inoculating needle. The slant was then incubated at 37°C for 48 hours. After incubation, the slant was taken out and observed for any change. The Bile Esculin Agar used in the test was used as a selective and differential medium. The bile that was in the medium was selective for streptococci and enterococci and inhibited the growth of Gram-positive staphylococci (Atlas & Snyder, 2015). The test differentiated between Streptococcus bovis group and Enterococcus from other streptococci based on the bacteria’s ability to hydrolyze esculin in the presence of bile. When esculin is hydrolyzed the byproduct reacts with ferric citrate and forms a dark precipitate on the medium (Atlas & Snyder, 2015). A Bile Esculin-positive result is if the media darkens around the bacteria and a negative result is if there is no darkening of the
The Bile Esculin test was performed on a Bile Esculin Agar slant by inoculating the slant in a zig-zag pattern and was stabbed using an inoculating needle. The slant was then incubated at 37°C for 48 hours. After incubation, the slant was taken out and observed for any change. The Bile Esculin Agar used in the test was used as a selective and differential medium. The bile that was in the medium was selective for streptococci and enterococci and inhibited the growth of Gram-positive staphylococci (Atlas & Snyder, 2015). The test differentiated between Streptococcus bovis group and Enterococcus from other streptococci based on the bacteria’s ability to hydrolyze esculin in the presence of bile. When esculin is hydrolyzed the byproduct reacts with ferric citrate and forms a dark precipitate on the medium (Atlas & Snyder, 2015). A Bile Esculin-positive result is if the media darkens around the bacteria and a negative result is if there is no darkening of the