Proteins are known as the building blocks of cells in all living organisms. Many hormones are made up of proteins and the digestive system, immune system and blood replies on proteins. Therefore, protein is an essential part of healthy diet, and integral to development of the body. The purpose of performing this lab is to analyzing the activity of TEM-1 B-lactamase and how it reacts with high temperatures, chaotropic salts, detergents and pH values.
INTRODUCTION:
Primary protein structure is sequence of chain of amino acids. Secondary protein structure is a regularly occurring structure and is mainly formed when a sequence of amino acids are linked between by hydrogen bonds. There are two types of stable secondary structures, the alpha helices …show more content…
The experimental results do agree with the known optimum conditions of TEM-1 B-lactamase. Most proteins functions ideally when the temperature is 37oC, and the pH is 7.4. When there is no competition between the salt ions and the charge residues of the proteins for water, and when there is no detergents to denature the protein even at a low concentration. There were few sources of errors during the experiment. During experiment 1, the enzymes were kept in the heating block for little too long. Heat exposed to the protein hardens the enzyme, and thus, makes it harder and longer for substrate to bind to it. In experiment 4, with pH value of 9, the TEM-1 enzyme and nitrocefin did react, however, the colour was so faint that it was seen, and therefore, was not recorded. Observing just the colour change to determine protein activity is not the best method because it is not a definitive answer. It’s a qualitative answer, based on a personal opinion and description. A different method could be using a spectrometer to see the wavelength of those colour changes, to quantify the color produced in a
INTRODUCTION:
Primary protein structure is sequence of chain of amino acids. Secondary protein structure is a regularly occurring structure and is mainly formed when a sequence of amino acids are linked between by hydrogen bonds. There are two types of stable secondary structures, the alpha helices …show more content…
The experimental results do agree with the known optimum conditions of TEM-1 B-lactamase. Most proteins functions ideally when the temperature is 37oC, and the pH is 7.4. When there is no competition between the salt ions and the charge residues of the proteins for water, and when there is no detergents to denature the protein even at a low concentration. There were few sources of errors during the experiment. During experiment 1, the enzymes were kept in the heating block for little too long. Heat exposed to the protein hardens the enzyme, and thus, makes it harder and longer for substrate to bind to it. In experiment 4, with pH value of 9, the TEM-1 enzyme and nitrocefin did react, however, the colour was so faint that it was seen, and therefore, was not recorded. Observing just the colour change to determine protein activity is not the best method because it is not a definitive answer. It’s a qualitative answer, based on a personal opinion and description. A different method could be using a spectrometer to see the wavelength of those colour changes, to quantify the color produced in a