System Suitability Results For Lamivudine, Zidovudine And Nevirapine

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Table 4.2 System suitability results for Lamivudine, Zidovudine and Nevirapine
Parameters Lamivudine Zidovudine Nevirapine
Retention time 2.327 2.864 5.608
Area 212473 338930 228426
USP Plate count 2715.09 3350.07 4979.51
USP Tailing 1.38 1.32 1.18

4.3.1.2 Linearity
A linear correlation coefficient factor was obtained between the concentrations of Lamivudine, Zidovudine and Nevirapine versus the peak area. The calibration curves were linear for concentrations between 15-150 µg/mL. The linearity of the calibration curves was evaluated by the values of the correlation coefficients (r2). The correlation coefficients were 0.999 for Lamivudine, 0.999 for Zidovudine and 0.999 for Nevirapine. The results of the linearity experiment are listed in
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Figure 4.6: A typical HPLC chromatogram of LOD for Lamivudine, Zidovudine and Nevirapine Figure 4.7: A typical HPLC chromatogram of LOQ for Lamivudine, Zidovudine and Nevirapine
Table 4.7: LOD & LOQ results for Lamivudine, Zidovudine and Nevirapine
Drug LOD Concentration
(in µg/mL) S/N Ratio Value LOQ Concentration (in µg/mL) S/N Ratio Value
Lamivudine 0.315 2.98 0.945 9.98
Zidovudine 0.405 2.97 1.080 9.97
Nevirapine 0.600 2.98 2.1 9.97

4.3.1.6 Robustness
Deliberate changes in the method, i.e., changes in the flow rate and the wavelength, did not significantly affect the peak tailing, the theoretical plates (Table 4.8).
Table 4.8: Robustness results for Lamivudine, Zidovudine and

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