The alkaloid content was determined gravimetrically using [19].
101 Five gram of each sample was weighed using a weighing balance and dispersed into 50 ml of 10% acetic
102 acid solution in ethanol. The mixture was then allowed to stand for about 4 h before it is filtered. The
103 filtrate was evaporated to one quarter of its original volume on hot plate. Concentrated ammonium
104 hydroxide was added drop wise in order to precipitate the alkaloids. A pre-weighed filter paper was used
105 to filter off the precipitate and it was washed with 1% ammonium hydroxide solution. The filter paper
106 containing the precipitate was dried in an oven at 60oC for 30 min, transferred into desiccators to cool
107 and then reweighed until a constant weight
101 Five gram of each sample was weighed using a weighing balance and dispersed into 50 ml of 10% acetic
102 acid solution in ethanol. The mixture was then allowed to stand for about 4 h before it is filtered. The
103 filtrate was evaporated to one quarter of its original volume on hot plate. Concentrated ammonium
104 hydroxide was added drop wise in order to precipitate the alkaloids. A pre-weighed filter paper was used
105 to filter off the precipitate and it was washed with 1% ammonium hydroxide solution. The filter paper
106 containing the precipitate was dried in an oven at 60oC for 30 min, transferred into desiccators to cool
107 and then reweighed until a constant weight