Survival Time:
Table 1 showsthe effect of different extracts of Punica grantum L and Ziziphus mauritiana on mean survival time and % ILS against EAC. In the EAC control group, the mean survival time was 14 days and it increased significantly to 23 and 21 days with ethanol extract of combination ofZiziphus mauritiana andPunica grantum L ZP (E) and ethanol extract Punica grantum L P(E) of treated groups respectively. The mean survival time in 5-Flurouracil treated mice found to be 26 days ( r < 0.001). The %ILS was found to be 63.38 %, 52.11%, 84.51% in EAC induced animals treated with ZP (E), P (E) and 5-Flurouracil respectively when compared with vehicle treated cancerous animals.
Table1: Effect of different extracts of Punica grantum L and …show more content…
Figure 2 show the percentage decrease in the body weight after treatment with different extracts of Punica grantum L and Ziziphus mauritiana on day 10. The percentage decrease was found to be 41.46%, 50.47% and 64.77% in EAC induced animals treated with P (E), ZP(E) and 5-Flurouracil respectively when compared with vehicle treated cancerous animals. ZP (E) significantly (r< 0.001, as compared with EAC control; z< 0.001, x< 0.05, as compared with 5-fluorouracil treated group.
n = 6, Values are mean ±S.E.M, one way ANOVA followed by Dunnet's multiple comparison test. P values: r< 0.001, as compared with EAC control; z< 0.001,y< 0.01 as compared with 5-fluorouracil treated group.
Hematological …show more content…
The total WBC count found significantly increased in the EAC control group when compared with the normal group (c < 0.001). Ethanol and aqueous extracts of combination ofZiziphus mauritiana andPunica grantum L (ZP) as well as ethanol extract of Punica grantum L P(E), showed activity at par with 5-Fluorouracil as standard and these differences were statistically non-significant for ethanol extract of ZP and y< 0.001) decreased as compared to normal group. Treatment with ZP(E) 200 mg/kg showed significant (r < 0.001) increase in the RBC count and Hb content when compared to the EAC control group, and restored these values towards normal [ Table 2 ].
Table 2: Effect of different extracts of Punica grantum L and Zizipus mauritiana on hematological parameters in EAC tumor bearing mice.
TREATMENT GROUP RBC COUNT (x 106/mL) WBC COUNT (x 103/mL) Hb (g%)
NORMAL 5.68 ± 0.11 6.96 ± 0.18 15.99 ± 0.39
EAC 2.17 ± 0.1 c 18.58 ± 0.55 c 7.07 ± 0.42 c
5-FU 4.65 ± 0.25 r 6.50 ± 0.34r 12.29 ± 0.56 br
P-E 3.95 ± 0.34 bq 9.94 ± 0.70 ary 9.74 ± 0.76
Table 1 showsthe effect of different extracts of Punica grantum L and Ziziphus mauritiana on mean survival time and % ILS against EAC. In the EAC control group, the mean survival time was 14 days and it increased significantly to 23 and 21 days with ethanol extract of combination ofZiziphus mauritiana andPunica grantum L ZP (E) and ethanol extract Punica grantum L P(E) of treated groups respectively. The mean survival time in 5-Flurouracil treated mice found to be 26 days ( r < 0.001). The %ILS was found to be 63.38 %, 52.11%, 84.51% in EAC induced animals treated with ZP (E), P (E) and 5-Flurouracil respectively when compared with vehicle treated cancerous animals.
Table1: Effect of different extracts of Punica grantum L and …show more content…
Figure 2 show the percentage decrease in the body weight after treatment with different extracts of Punica grantum L and Ziziphus mauritiana on day 10. The percentage decrease was found to be 41.46%, 50.47% and 64.77% in EAC induced animals treated with P (E), ZP(E) and 5-Flurouracil respectively when compared with vehicle treated cancerous animals. ZP (E) significantly (r< 0.001, as compared with EAC control; z< 0.001, x< 0.05, as compared with 5-fluorouracil treated group.
n = 6, Values are mean ±S.E.M, one way ANOVA followed by Dunnet's multiple comparison test. P values: r< 0.001, as compared with EAC control; z< 0.001,y< 0.01 as compared with 5-fluorouracil treated group.
Hematological …show more content…
The total WBC count found significantly increased in the EAC control group when compared with the normal group (c < 0.001). Ethanol and aqueous extracts of combination ofZiziphus mauritiana andPunica grantum L (ZP) as well as ethanol extract of Punica grantum L P(E), showed activity at par with 5-Fluorouracil as standard and these differences were statistically non-significant for ethanol extract of ZP and y< 0.001) decreased as compared to normal group. Treatment with ZP(E) 200 mg/kg showed significant (r < 0.001) increase in the RBC count and Hb content when compared to the EAC control group, and restored these values towards normal [ Table 2 ].
Table 2: Effect of different extracts of Punica grantum L and Zizipus mauritiana on hematological parameters in EAC tumor bearing mice.
TREATMENT GROUP RBC COUNT (x 106/mL) WBC COUNT (x 103/mL) Hb (g%)
NORMAL 5.68 ± 0.11 6.96 ± 0.18 15.99 ± 0.39
EAC 2.17 ± 0.1 c 18.58 ± 0.55 c 7.07 ± 0.42 c
5-FU 4.65 ± 0.25 r 6.50 ± 0.34r 12.29 ± 0.56 br
P-E 3.95 ± 0.34 bq 9.94 ± 0.70 ary 9.74 ± 0.76