This hospital-based case-control study was conducted on 110 consecutively newly diagnosed LP patients and 120 apparently healthy, age and sex-matched, control subjects. The control group consisted of participants referred for non-inflammatory dermatological conditions such as chronic hair loss or coming with their relatives. They had no oral mucosal, systemic or autoimmune diseases, and had no history of LP. All subjects were recruited from the outpatient clinic of the Department of Dermatology, Faculty of Medicine and the Department of Oral Medicine, Diagnosis and Periodontology, Faculty of Oral and Dental Medicine, Cairo University between September 2014 and August 2015.
Diagnosis of LP was confirmed based on the typical clinical presentation. Patients were subgrouped into two main groups; patients with only OLP lesions or patients with mainly CLP lesions (generalized, localized or associated with OLP). Patients on phototherapy, or suffering from any cancer, or receiving systemic treatment within the last 4 weeks have been excluded from the study. …show more content…
All subjects signed a written informed consent before enrolment.
Three ml venous blood sample was taken after fasting overnight (12 h) from each subject for detection of MTHFR gene polymorphism by PCR-RFLP technique and for measurement of the following laboratory investigations: plasma cholesterol, triglycerides, high-density lipoprotein (HDL), Presence of hypertension has been assessed. Hcy and folic acid were measured by ELISA kit supplied by (CUSABIO) and (MyBioSource) respectively according to manufacturer’s
Diagnosis of LP was confirmed based on the typical clinical presentation. Patients were subgrouped into two main groups; patients with only OLP lesions or patients with mainly CLP lesions (generalized, localized or associated with OLP). Patients on phototherapy, or suffering from any cancer, or receiving systemic treatment within the last 4 weeks have been excluded from the study. …show more content…
All subjects signed a written informed consent before enrolment.
Three ml venous blood sample was taken after fasting overnight (12 h) from each subject for detection of MTHFR gene polymorphism by PCR-RFLP technique and for measurement of the following laboratory investigations: plasma cholesterol, triglycerides, high-density lipoprotein (HDL), Presence of hypertension has been assessed. Hcy and folic acid were measured by ELISA kit supplied by (CUSABIO) and (MyBioSource) respectively according to manufacturer’s