Stereotactic Injection: Anisomycin (Sigma-Aldrich, St. Louis, MO) was dissolved in an equimolar concentration of HCl, adjusted to pH 7.2 with NaOH, and brought to the final concentrations of 64 μg/μl in physiological saline. Mice received bilateral injections of Anisomycin (ANI; 0.2 μl per side) or vehicle (0.2 μl of physiological saline) per side using a double infusion needle connected via tubing to two Hamilton syringes (Reno, NV) operated by a Cole Parmer (Vernon Hills, IL) multi-syringe infusion pump. Injections were performed immediately after each contextual fear conditioning training session or after 6 hours.
siRNA-mediated focal knockdown of Homer 3 was first assayed in hippocampal and cortical neurons in primary culture 48 and 72
siRNA-mediated focal knockdown of Homer 3 was first assayed in hippocampal and cortical neurons in primary culture 48 and 72