Santoor Movement Experiment

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Introduction Movement speed of Stentor is dependent upon the temperature of water in the environment surrounding Stentor. Stentor is a member of the Phylum Ciliophora. Stentor are ciliate unicellular protozoa which is one the largest singled celled organisms (Webb, 2007) Stentor are found in waters that are calm usually near the surface on different plants (Webb, 2007). Stentor use cilia to consume food and movement. Stentor are ectothermic organisms, which means that Stentor body temperature is controlled by their environment (Hickman, Kats, Keen 2013). This lab examines how the movement of Stentor may be effected while placed in 5ºC and 20ºC water over a period of time. If locomotion in Stentor is to be delayed or sped-up then the temperature …show more content…
Materials & Methods This experiment required the use of a depression slide with grid lines. Vaseline was used to create a ring around the depression on the slide to prevent Stentor from seeping out. It was important to avoid applying Vaseline inside the depression of the slide as it may cause Stentor to become trapped. After completing the preparation to observe Stentor a pipette was used to extract three to four droplets of liquid that contained Stentor. Once the Stentor has been transferred from the pipette to the depression of the slide, a coverslip was applied. Next a finger bowl was filled with cold water and some ice was added to the water to bring the temperature to 5ºC. A thermometer was used several times to observe the temperature for accuracy. Once the water reached 5ºC the slide containing Stentor was placed into the water and observed with a dissecting microscope. One individual kept time as another observed how many lines were crossed in one minute. Patterns of Stentor remaining in one cube did not count as multiple lines crossed. In order to conclude that Stentor traveled a line means it had to travel over to …show more content…
In the first trial of 5ºC water Stentor were able to travel 10 complete lines in one minute. Eight lines were crossed in the second trail and 5 lines in the final trial. Overall, the range of the three trials was 5, with 5 as the lowest and 10 as the maximum amount of lines crossed. The mean or average amount of lines crossed in 5ºC water was 7.67 as shown in figure 1. The standard deviation was 4.84. Trial numbers increased sharply when Stentor were placed into warmer water. In the first trial of 20ºC water, Stentor traveled 24 lines in one minute. Stentor traveled 28 lines in the second trial and 27 lines in the final trial of 20ºC water results are listed in table 1. The range for the 20ºC water was a difference of 3 lines and the mean was 26.3 lines also shown in table 1. The standard deviation for 20ºC water was 2.08. The t-test concludes that the null hypothesis is false. With respect to the entire class, conclusively the range of the entire group’s data was 22 in 5ºC and 68 in 20ºC. The average number of lines crossed for the entire group in 5ºC water was 5.36 lines and in 20ºC was 43 lines both results are detailed in fig. 1.3 and 1.4. The standard deviation for 5ºC was 4.84 and for 20ºC 17.42As outlined in fig. 1 the most individuals observed Stentor moving 0-5 in 5ºC water and in fig 1.4 most observations show Stentor traveling 35-49 lines in 20ºC water. This

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