Catalase Test Paper

Through careful evaluation in all the test that were successfully completed it is safe to say that there is indeed Staphylococcus aureus within the throat. In the collection and isolation of the mixed culture from the nose and throat there were seven different test that aided in conformation of these two samples, which was the MSA fermentation plates, the beta hemolysis of the blood and DNA agar plate, the coagulase test, Antimicrobial substibility, the characteristics that were viewed under the microscope, and the catalase test. Out of these test, the determining factor in detecting positive or negative confirmation for Staphylococcus aureus is through the coagulase test which is further explained in Table 1 and figure 5, the results were positive (Kateete). …show more content…
This further explains that the individual is most likely a carrier for the throat, however not a carrier for Staphylococcus aureus in the nose. The Blood and DNA agar plate was tested to see if there was a particular enzyme that Staphylococcus aureus has as well (Kateete). Although, all samples in the blood agar plate was negative as indicated in the 1st table and in figure 3, the DNA agar plate all six were positive. As specified “if the isolates that are DNase-positive and positive for at least one other test it should be considered potential S. aureus” (Kiser 79). Also, the Kirby-Bauer disk diffusion test displayed resistance to four different antibiotics, one which is penicillin, a big indication due to S. aureus having a beta lactamase enzyme and being able to a bind penicillin protein together (Kateete). However, if that’s not enough evidence there’s always a better look under the microscope which indeed contained Staphylococcus aureus characteristics as shown in figure 7. In addition, the catalase test verified that it was indeed Staph by the bubbling which formed from the hydrogen (Kiser