Background:
Oral squamous cell carcinoma is a challenging oncology problem. A reliable biomarker for invasion and metastasis in oral cancer patients remains to be more clarified. β-catenin acts as a structural protein at cell-cell adherence junctions and as a transcription activator mediating Wnt signal transduction. Altered β-catenin expression has been associated with loss of cell differentiation and acquisition of an invasive phenotype. Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) is an abundant neuronal deubiquitinating enzyme that has also been suggested to possess ligase activity and/or stabilize ubiquitin monomers in vivo. Recent evidences implicate dysregulation of UCHL1 in the pathogenesis and progression of several human cancers. …show more content…
(2)To evaluate, compare, and correlate the immunohistochemical pattern of expressions and intracellular locations of β-catenin and UCHL1 in oral squamous cell carcinoma. (3) To correlate β-catenin and UCHL1 expressions with different oral squamous cell carcinoma Bryne’s grades.
Materials and methods:
This retrospective study is concerned with evaluation of clinicopathological features of 30 oral squamous cell carcinoma formalin fixed paraffin blocks using two grading systems )WHO’s and Bryne’s grading systems). Then by using immunohistochemical procedure, the expression, subcellular localization, pattern of expression, and intensity of the stain of β-catenin and UCHL1 in oral squamous cell carcinoma were examined, and correlated with each other and with different histological grades of Bryne’s system. Abstract
III
Results:
The studied sample included 53.3% males and 46.7% females with a ratio of 1.14:1, with a wide age range of 27-85 years, but the higher number of OSCC was in 50-69 years age group. Most OSCC cases were in the tongue and with a significant ulcerative clinical presentation as
Oral squamous cell carcinoma is a challenging oncology problem. A reliable biomarker for invasion and metastasis in oral cancer patients remains to be more clarified. β-catenin acts as a structural protein at cell-cell adherence junctions and as a transcription activator mediating Wnt signal transduction. Altered β-catenin expression has been associated with loss of cell differentiation and acquisition of an invasive phenotype. Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) is an abundant neuronal deubiquitinating enzyme that has also been suggested to possess ligase activity and/or stabilize ubiquitin monomers in vivo. Recent evidences implicate dysregulation of UCHL1 in the pathogenesis and progression of several human cancers. …show more content…
(2)To evaluate, compare, and correlate the immunohistochemical pattern of expressions and intracellular locations of β-catenin and UCHL1 in oral squamous cell carcinoma. (3) To correlate β-catenin and UCHL1 expressions with different oral squamous cell carcinoma Bryne’s grades.
Materials and methods:
This retrospective study is concerned with evaluation of clinicopathological features of 30 oral squamous cell carcinoma formalin fixed paraffin blocks using two grading systems )WHO’s and Bryne’s grading systems). Then by using immunohistochemical procedure, the expression, subcellular localization, pattern of expression, and intensity of the stain of β-catenin and UCHL1 in oral squamous cell carcinoma were examined, and correlated with each other and with different histological grades of Bryne’s system. Abstract
III
Results:
The studied sample included 53.3% males and 46.7% females with a ratio of 1.14:1, with a wide age range of 27-85 years, but the higher number of OSCC was in 50-69 years age group. Most OSCC cases were in the tongue and with a significant ulcerative clinical presentation as