The methodology the authors in this paper utilize to closely analyze the genomic content and the evolutionary history of Asgard archaea lineages is referred to as shot-gun DNA sequencing. Next Generation Sequencers (NGS) were used to expedite shot-gun sequencing. Shot-gun sequencing is a key method used by geneticists and biochemists in sequencing long DNA strands. In this method, long sequences are separated into shorter sequences and then bonded together again to give the complete sequence. The shorter sequences are identified as reads. The reads are classified through their function, this depends on if it is an end-read or a read. These reads are then reassembled using a computer program for sequence assembly in which they carefully aligned
