August 22, 2014
Dr. Caryn Ito
Associate Scientist
Sprott Centre for Stem Cell Research
Ottawa Hospital Research Institute
Ottawa, ON
Dear Dr. Ito:
Enclosed is my work-term report entitled Summer 2014 Work-Term Report: My experiences as CO-OP student working in the Ito/Stanford Lab at the Ottawa Hospital Research Institute. It is a description of the workplace, sepsis and the Ito/Stanford Lab’s sepsis project, my role in the project, and the skills that I have obtained during this placement. This report is submitted to fulfill the University of Ottawa’s CO-OP requirements. It is the culmination of my first work term.
Thank you for this opportunity,
Daniella Latty
Summer 2014 Work-Term Report:
My experiences as CO-OP student working in the Stanford Lab at the Ottawa Hospital Research Institute
¬¬
Daniella Latty
6879808
Biomedical Mechanical Engineering
University of Ottawa
August 22, 2014 Executive Summary
This report is a summary …show more content…
This project seeks to develop a novel humanized murine model of sepsis to determine the efficacy of a Human Umbilical Cord Perivascular Cell-derived treatment for the disease. My placement involved the testing of three potential mouse models to find the model most optimal in terms of modeling human sepsis: NOD scid gamma (NSG) mice repopulated with 1x105 Lineage negative (Lin-) Umbilical Cord Blood (UCB) Cells, NSG mice transgenic for human IL3, GM-CSF, and SCF, repopulated with 1x105 Lin- UCB cells, and NSG mice repopulated with 5x106 UCB mononuclear cells. WHY WERE THESE MODELS PICKED, WHAT ARE SOME BENEFITS? These humanized mouse models involved a cecal ligation and puncture procedure to induce sepsis. Peripheral blood was collected from the mice at endpoint. This blood was used to analyse their gene expression, and thus determine which mouse model most closely resembles human sepsis according to gene expression
Dr. Caryn Ito
Associate Scientist
Sprott Centre for Stem Cell Research
Ottawa Hospital Research Institute
Ottawa, ON
Dear Dr. Ito:
Enclosed is my work-term report entitled Summer 2014 Work-Term Report: My experiences as CO-OP student working in the Ito/Stanford Lab at the Ottawa Hospital Research Institute. It is a description of the workplace, sepsis and the Ito/Stanford Lab’s sepsis project, my role in the project, and the skills that I have obtained during this placement. This report is submitted to fulfill the University of Ottawa’s CO-OP requirements. It is the culmination of my first work term.
Thank you for this opportunity,
Daniella Latty
Summer 2014 Work-Term Report:
My experiences as CO-OP student working in the Stanford Lab at the Ottawa Hospital Research Institute
¬¬
Daniella Latty
6879808
Biomedical Mechanical Engineering
University of Ottawa
August 22, 2014 Executive Summary
This report is a summary …show more content…
This project seeks to develop a novel humanized murine model of sepsis to determine the efficacy of a Human Umbilical Cord Perivascular Cell-derived treatment for the disease. My placement involved the testing of three potential mouse models to find the model most optimal in terms of modeling human sepsis: NOD scid gamma (NSG) mice repopulated with 1x105 Lineage negative (Lin-) Umbilical Cord Blood (UCB) Cells, NSG mice transgenic for human IL3, GM-CSF, and SCF, repopulated with 1x105 Lin- UCB cells, and NSG mice repopulated with 5x106 UCB mononuclear cells. WHY WERE THESE MODELS PICKED, WHAT ARE SOME BENEFITS? These humanized mouse models involved a cecal ligation and puncture procedure to induce sepsis. Peripheral blood was collected from the mice at endpoint. This blood was used to analyse their gene expression, and thus determine which mouse model most closely resembles human sepsis according to gene expression