The sciatic nerve used for the experiment was removed from a bullfrog that had been double-pithed. This means that the connection between the frog’s spinal cord and brain was severed. The result being that the frog could no longer feel the sensation of pain and that its muscle reflexes no longer functions. The frog’s skin was cut and pulled all the way down its body from the abdomen and removed from the legs. Its thigh muscles were then separated in order to expose the nerve. After its removal it was placed in a beaker filled with amphibian perfusion fluid (Frog Ringer’s) to prevent it from becoming too dry and dying before
