Sample Examination Of Water : Most Probable Number Test And Membrane Filtration Method

1902 Words Sep 18th, 2014 8 Pages
CEE 467 – ENVIRONMENTAL MICROBIOLOGY
NAME: ERICK PEREIRA DANTAS
DATE: SEPTEMBER 4th, 2014
PROFESSOR: DR. MORTEZA ABBASZADEGAN
TA: LAUREN MCBURNETT

LABORATORY #2 – BACTERIOLOGICAL EXAMINATION OF WATER: MOST PROBABLE NUMBER TEST AND MEMBRANE FILTRATION METHOD

ABSTRACT: The most probable number (MPN) test and the membrane filter (MF) method have as goal to detect and estimate the presence of coliforms in a sample. Their importance in the field of environmental engineering is to support the start of the analysis for possible biological contamination in water for causative microorganisms of waterborne disease. For they use coliforms they are cheaper and faster than others. This experiment had positive results. The colonies were counted and the average final was 455 CFU per 100 ml of the sample used. During the MPN test the coliforms produced gas due to the lactose in the medium and some tubes presented high turbidity due acid production. And, in the MF the colonies were identified by their distinctive color. In brief, these techniques are essential for environmental investigations related to microbiological contamination of water, particularly in drinking water.

1 INTRODUCTION In this laboratory were performed two techniques of bacteriological examination: the most probable number (MPN) test and the membrane filtration (MF) technique. Stocks of E. coli were utilized in both processes as indicator organisms. Indicator organisms are microorganisms used to…

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