Salvinia molesta is an invasive species that has consumed many bodies of water in the states of Louisiana, Texas, Alabama, and Colorado (McFarland et al. 2004). The invasive species originated from South America and the first place it was transferred to was Australia (Room 1983). Eichhornia crassipes also originated from South America (Gopal and Kanta 1981). Lemna sp. is native to many places around the world (Azeez and Sabbar 2012).
The extracts were created from E. crassipes roots and shoots, and Lemna sp. The plants were spun in a salad spinner ten times. Each plant species had to accumulate a weight of 640 grams. The plants was weighted on a balance. Once the plants were weighted then the plant material was grinded with a blender. The E. …show more content…
The beaker was labeled with plant species/part and diH2O. The grounded-up plants were soaked in diH2O for 24 hours in a refrigerator. After 24 hours each plant sample was filtered through 4 layers of cheese cloth and then filtered through filter paper twice. The filter paper that was used was Whatman Qualitative Grade 1 Plain Circles, and multiple filters were used during the process. The samples was filtered through 0.45 um Millipore filters (EMD Millipore Stericup Sterile vacuum filter units). Once all filtered processes were complete the samples was covered with parafilm and store in -4degree C freezer. Then nine 2L flask was labeled the following: ECR20,ECR100,ECR200,ECS20,ECS100,ECS200, L20,L100,L200. The ECR represented E. crassipes root, ECS represented E. crassipes shoot, L represented Lemna sp. Then each extract was brought to the volume of 1280 ml. In the sample of 20 ppt 80ml of extract was added to 1920 ml diH2O in a 2 L flask. In sample of 100 ppt 400 ml of extract was added to 1600 ml of diH2O in a 2 L flask. For the sample 200 ppt 800 ml of the extract was added to 1200 ml of diH2O in a 2 L flask. This was done for E. …show more content…
crassipes root extract, Lemna sp. extract. For the treatments listed above and including the control (C) twenty small containers were labeled with the treatment or control and label a replicate number. The replicate number would be ECR20-1, ECR20-2 all the way through 20 then label ECR100-1,ECR100-2 all the way through 20 this was done for all treatments. The control was Salvinia molesta with no extract included. The sample size is twenty containers a treatment. After all containers were labeled then S. molesta was spun ten times in a salad spinner. 3 grams of S. molesta was weighted out for replicated treatments including the control. The wet weight of the S. molesta was recorded and placed in the appropriate container. 100 ml of each extract solution was added to the appropriate labeled containers. The containers were randomly placed on racks in rows and the containers were placed evenly apart. The RANDOM.ORG was used for randomization. The experiment was then run for seven days. After seven days each
The extracts were created from E. crassipes roots and shoots, and Lemna sp. The plants were spun in a salad spinner ten times. Each plant species had to accumulate a weight of 640 grams. The plants was weighted on a balance. Once the plants were weighted then the plant material was grinded with a blender. The E. …show more content…
The beaker was labeled with plant species/part and diH2O. The grounded-up plants were soaked in diH2O for 24 hours in a refrigerator. After 24 hours each plant sample was filtered through 4 layers of cheese cloth and then filtered through filter paper twice. The filter paper that was used was Whatman Qualitative Grade 1 Plain Circles, and multiple filters were used during the process. The samples was filtered through 0.45 um Millipore filters (EMD Millipore Stericup Sterile vacuum filter units). Once all filtered processes were complete the samples was covered with parafilm and store in -4degree C freezer. Then nine 2L flask was labeled the following: ECR20,ECR100,ECR200,ECS20,ECS100,ECS200, L20,L100,L200. The ECR represented E. crassipes root, ECS represented E. crassipes shoot, L represented Lemna sp. Then each extract was brought to the volume of 1280 ml. In the sample of 20 ppt 80ml of extract was added to 1920 ml diH2O in a 2 L flask. In sample of 100 ppt 400 ml of extract was added to 1600 ml of diH2O in a 2 L flask. For the sample 200 ppt 800 ml of the extract was added to 1200 ml of diH2O in a 2 L flask. This was done for E. …show more content…
crassipes root extract, Lemna sp. extract. For the treatments listed above and including the control (C) twenty small containers were labeled with the treatment or control and label a replicate number. The replicate number would be ECR20-1, ECR20-2 all the way through 20 then label ECR100-1,ECR100-2 all the way through 20 this was done for all treatments. The control was Salvinia molesta with no extract included. The sample size is twenty containers a treatment. After all containers were labeled then S. molesta was spun ten times in a salad spinner. 3 grams of S. molesta was weighted out for replicated treatments including the control. The wet weight of the S. molesta was recorded and placed in the appropriate container. 100 ml of each extract solution was added to the appropriate labeled containers. The containers were randomly placed on racks in rows and the containers were placed evenly apart. The RANDOM.ORG was used for randomization. The experiment was then run for seven days. After seven days each