Salvinia Molest

The plants were collected using aquatic nets, shovels, and by hand. The plants collected was then put into a bucket or bag and was label with the collection site and the date with a sharpie. If buckets was a storage for plants then a couple of inches of water was added. Once the plants were collected they were not exposed to the sun because they could desiccate. All storage containers for the plants were sealed secure before leaving the collection site. Forty five clean buckets were labeled CON for control which was Salvinia molesta alone, EC for Eichhornia crassipes with Salvinia molesta, or L for Lemna sp. with Salvinia molesta. Each bucket from groups CON, EC, and L was also labeled one through fifteen. These buckets were taken to RRWMI Field station by LSUS campus where they …show more content…
A balance was used to find the wet weight and a ruler was used to find the length and width of the leaves, the height of the shoot, and the length of roots. The plants were positioned in their correct number bag. Petri dishes were labeled L 1-15 and the diameter of the dishes was recorded. The Lemna sp. was spun in the salad spinner ten times. The surface of the petri dish was filled with Lemna sp., and each plant mass was assigned a number. Wet weight, leaf length and width of five leaves on each mass was recorded for Lemna sp. These masses were returned to the petri dishes. Sandwich bags were labeled CON 1-15, EC 1-15, and L 1-15 and S. molesta was spun ten times with the salad spinner. A clump of S. molesta was taken to fill the surface area of petri dish. Once filled the wet weight, leaf length and for five leaves per clump was recorded, and was placed in the designated numbered bags. The plants, the masses, and the clumps were transported to RRWMI field station where they were added to their designated buckets.