Discussion
The purpose of this experiment was to examine the relationship between salt stress and peroxidase activities in corn (Zea mays). To do this, we watered germinating corn seeds with various salt solutions.
For our first part of out experiment, we wanted to see what effect NaCl would have on peroxidase activities in corn. We watered week old corn with either 25mM or 75mM NaCl solutions. We found the band intensities for 75mM treatment all either went up or stayed the same as the 25mM treatment (fig.1). The peroxidases that went up (px 9.2, px 8.6, px 8.4, and px 8.2) may have gone up because these peroxidases play a role in relieving salt stress from NaCl.
It has been found before that corn will cease leaf growth if exposed to saline …show more content…
20mM) in its soil during its growth (Sivasankaramoorthy 2014). When corn seeds are exposed to salt concentrations of 20mM of NaCl and 20mM of CaCl2, the seeds grow at a much faster rate. A seed water with these concentrations show that after 15 days of growth, they will have grown approximately 1cm longer than a seed watered with tap water. From this we believe that the amount of 25mM NaCl could be beneficial for the plants growth. We see this in px 9.2 and px 8.6 because these peroxidases are believed to be leaf growth suppressors as stated previously, but they are lowered in the 25mM NaCl concentration showing that they are not as active anymore.
For our second part of out experiment, we watned to see what effect different types of salt would have on peroxidase activities in corn. We watered out week old corn with either 50mM NaCl or 50mM CaCl2. We found that peroxidases 8.29, 8.49, 8.95, and 9.56. in plants watered with NaCl had band intensities that went down when compared to the control. However, these same peroxidases went up (excluding px 9.56) in the CaCl2 treated plants. We believe that this could be caused by way the difference in ions in the salts affect water transport and or the chemical compounds inside the
The purpose of this experiment was to examine the relationship between salt stress and peroxidase activities in corn (Zea mays). To do this, we watered germinating corn seeds with various salt solutions.
For our first part of out experiment, we wanted to see what effect NaCl would have on peroxidase activities in corn. We watered week old corn with either 25mM or 75mM NaCl solutions. We found the band intensities for 75mM treatment all either went up or stayed the same as the 25mM treatment (fig.1). The peroxidases that went up (px 9.2, px 8.6, px 8.4, and px 8.2) may have gone up because these peroxidases play a role in relieving salt stress from NaCl.
It has been found before that corn will cease leaf growth if exposed to saline …show more content…
20mM) in its soil during its growth (Sivasankaramoorthy 2014). When corn seeds are exposed to salt concentrations of 20mM of NaCl and 20mM of CaCl2, the seeds grow at a much faster rate. A seed water with these concentrations show that after 15 days of growth, they will have grown approximately 1cm longer than a seed watered with tap water. From this we believe that the amount of 25mM NaCl could be beneficial for the plants growth. We see this in px 9.2 and px 8.6 because these peroxidases are believed to be leaf growth suppressors as stated previously, but they are lowered in the 25mM NaCl concentration showing that they are not as active anymore.
For our second part of out experiment, we watned to see what effect different types of salt would have on peroxidase activities in corn. We watered out week old corn with either 50mM NaCl or 50mM CaCl2. We found that peroxidases 8.29, 8.49, 8.95, and 9.56. in plants watered with NaCl had band intensities that went down when compared to the control. However, these same peroxidases went up (excluding px 9.56) in the CaCl2 treated plants. We believe that this could be caused by way the difference in ions in the salts affect water transport and or the chemical compounds inside the