Salmonella Lab Report

Belong to the Enterobacteriaceae family, Salmonella is responsible for thousands of hospitalization across the United States every year. Due to their high pathogenecity, even a small amount of Salmonella in food or water could cause various symptoms, including fever, diarrhea and abdominal cramps. While most people who experience these symptoms can be recovered within a few days, some need to be hospitalized due to severe diarrhea and high fever. Hence, salmonella contamination is a very serious problem and that detection of Salmonella at low level is important in food products. Through this lab, we will get to grow Salmonella from contaminated sample and perform a series of test to confirm its presence.
In this lab, Salmonella need to be enriched in a lactose broth first …show more content…
Through the use pre-enrichment media that consists of lactose broth, any microorganisms that are not Salmonella wouldn’t be able to survive because of the drop off in pH. After the Salmonella was allowed to growth during the incubation period, Tetrathionate Brilliant Green Both (TBGB) was used to promote the growth of Salmonella, while inhibiting the growth of other microorganisms, such as Gram-positive bacteria as well as Gram-negative bacilli. After the enrichment period, Brilliant Green Agar (BGA) was used as a selective medium for Salmonella, as microbes that are able to ferment lactose and sucrose will appear with yellow butt and slant, while Salmonella will appear red. Finally, the suspected Salmonella colony will then be inoculate in TSI and LIA agar slants in order to be confirm. TSI slants is a test that is use to distinguish microbes that ferments different types of sugar with