In addition, the first test will require a 0.90% saline solution maintained at ambient temperature and 7.0 pH in which the control group will be stored. A 0.90% saline solution maintained at 37.0 degrees Celsius and pH 7.15 will be the solution in which the experimental group will be stored. A pH of 7.15 was chosen because this is the pH of resting human muscle cells (University of Mississippi Medical Center, n.d.). For the second test, a total of eleven of each type of ligament, including SRT protein ligaments, ACL ligaments derived from autologous tissue, and native ACL ligaments will be required. The ligaments from the cadavers, both autologous and native ligaments, must be less than ten days old, to prevent the external variable of putrefaction from altering the accuracy of the data. The third test will require forty mice and twenty SRT protein ACL
In addition, the first test will require a 0.90% saline solution maintained at ambient temperature and 7.0 pH in which the control group will be stored. A 0.90% saline solution maintained at 37.0 degrees Celsius and pH 7.15 will be the solution in which the experimental group will be stored. A pH of 7.15 was chosen because this is the pH of resting human muscle cells (University of Mississippi Medical Center, n.d.). For the second test, a total of eleven of each type of ligament, including SRT protein ligaments, ACL ligaments derived from autologous tissue, and native ACL ligaments will be required. The ligaments from the cadavers, both autologous and native ligaments, must be less than ten days old, to prevent the external variable of putrefaction from altering the accuracy of the data. The third test will require forty mice and twenty SRT protein ACL