proteins like NSF, SNAPs (soluble NSF attachment proteins), and SNAREs including SNAP25, VAMP and syntaxin. With these identified proteins, Rothman laboratory developed the SNARE hypothesis describing a model of SNAREs involvement in vesicle targeting and fusion. Their model explains that a transport vesicle (v-SNARE) and a target membrane (t-SNARE) with the aid of NSF, SNAPs and other yet unknown proteins bring the v-SNARE in close proximity to the t-SNARE facilitating membrane fusion (Sollner et al., 1993). It is now widely accepted that SNAREs serve as the core machineries necessary for vesicle targeting and fusion but are regulated by proteins like Sec1/MUNC18, Rab Ypt GTPases and others that binds to them (Rothman, 1994, Bonifacino and
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Porter et al., 1945 using cells derived from chick embryos tissues with the aid of electron microscopy examined into detail the structural outline of the cell and organelles including: mitochondrion, Golgi Bodies and endoplasmic reticulum (Porter et al., 1945). This then created an avenue for enquiry into how these compartments within the cell interacts, how molecules move from one compartment to the other. In order to address concerns surrounding trafficking of substances through the Golgi complex, Caro and Palade, 1964 injected guinea pigs with a DL-leucine radiolabelled physiological saline and then their pancreas were removed and examined at different time points (Caro and Palade, 1964). Their work showed that some of the radiolabelled substances located at the rough endoplasmic reticulum have moved into the Golgi complex after 20 min and then to the secretory granules leading to their secretion at the PM (Caro and Palade, 1964). These results provided a direct evidence of the role of the Golgi complex in secretion. Although, their results could not show how those substances were transferred from the ER to the Golgi, they did propose that small vesicles might mediate these process (Caro and Palade, 1964). This study
Porter et al., 1945 using cells derived from chick embryos tissues with the aid of electron microscopy examined into detail the structural outline of the cell and organelles including: mitochondrion, Golgi Bodies and endoplasmic reticulum (Porter et al., 1945). This then created an avenue for enquiry into how these compartments within the cell interacts, how molecules move from one compartment to the other. In order to address concerns surrounding trafficking of substances through the Golgi complex, Caro and Palade, 1964 injected guinea pigs with a DL-leucine radiolabelled physiological saline and then their pancreas were removed and examined at different time points (Caro and Palade, 1964). Their work showed that some of the radiolabelled substances located at the rough endoplasmic reticulum have moved into the Golgi complex after 20 min and then to the secretory granules leading to their secretion at the PM (Caro and Palade, 1964). These results provided a direct evidence of the role of the Golgi complex in secretion. Although, their results could not show how those substances were transferred from the ER to the Golgi, they did propose that small vesicles might mediate these process (Caro and Palade, 1964). This study