Intro
Rubisco is the most abundant protein on earth and is found in plants to aid the process of carbon fixation. Isolation of Rubisco from a mixture of protein allows it to perform at its optimal level because it has the highest purity. The three techniques used in this lab are salting out, Ion exchange, and SDS-page. After the last technique is carried out, Rubisco is in its purest form since purity is enhanced with each subsequent technique.
Salting out is the first isolation technique used in this lab. This technique requires an addition of ammonium sulfate into a solution containing a mixture of protein (Duong-Ly & Gabelli, 2014). Since Rubisco is negatively charged and the ammonium ion in water is positively charged, Rubisco and water
Rubisco is the most abundant protein on earth and is found in plants to aid the process of carbon fixation. Isolation of Rubisco from a mixture of protein allows it to perform at its optimal level because it has the highest purity. The three techniques used in this lab are salting out, Ion exchange, and SDS-page. After the last technique is carried out, Rubisco is in its purest form since purity is enhanced with each subsequent technique.
Salting out is the first isolation technique used in this lab. This technique requires an addition of ammonium sulfate into a solution containing a mixture of protein (Duong-Ly & Gabelli, 2014). Since Rubisco is negatively charged and the ammonium ion in water is positively charged, Rubisco and water