Introduction
Mungbean (Vigna radiata) is a leguminous crop which serves as an important food and cash crop which is cultivated in Asia, Africa, Australia, North and South America. It is a short duration crop and requires low farm inputs. The world’s production of mungbean is more than 6 million hectares per annum and has the highest production in Asia.
It is eaten as a vegetable and it is also processed into various products such as sweets, noodles and drinks. Mungbean is rich in easily digestible protein and micronutrients such as iron and zinc. (Akaerue et al., 2010; Kollarova et al., 2010). It is highly profitable rotation crop for Asian cereal production areas due to its high market demand which commands relatively good farm prices for …show more content…
The most effective control measure to prevent the infestation of bruchid in large scale storage is chemical control (Daglishet et al., 1993), but it has advantages which are not environment and consumer benign such as increment in storage cost, chemical exposure to consumers and farmers, and development of resistance to the targeted pest (Gbaye et al., 2011). The efficiency of bio-control methods using plant materials or parasitoids are available but seems to be limited (Brisibe et al., 2011, Soundararajan et al., 2012). The most efficient and efficient control measure of bruchids which is environment and consumer friendly is host …show more content…
Extraction of DNA could be done from any samples but fresh samples are the best for extraction for good quality DNA. There are many protocols in extracting DNA which are simple, quick, less laborious and less time consuming which produce high quality and quantity of DNA e.g. QIAGEN RNeasy.
There are different types of molecular markers and they are grouped by Semagn et al., 2016 based on their method of analysis (Hybridization-based molecular markers or PCR-based molecular markers).
Molecular markers are also used to detect the genomic structure of different organisms, genotypic variations such as insertions, mutations, deletions and single nucleotide polymorphisms, genome organization and evolutions (Jones et al., 2009). In many crop breeding programmes, these markers are used to track loci and genomic regions which are strongly linked with a large number of agronomic and disease resistance traits found in crop species (Varshney et., 2007). In this study, we applied single nucleotide polymorphism (SNP) markers which are described in detail.
Single Nucleotide Polymorphism
Mungbean (Vigna radiata) is a leguminous crop which serves as an important food and cash crop which is cultivated in Asia, Africa, Australia, North and South America. It is a short duration crop and requires low farm inputs. The world’s production of mungbean is more than 6 million hectares per annum and has the highest production in Asia.
It is eaten as a vegetable and it is also processed into various products such as sweets, noodles and drinks. Mungbean is rich in easily digestible protein and micronutrients such as iron and zinc. (Akaerue et al., 2010; Kollarova et al., 2010). It is highly profitable rotation crop for Asian cereal production areas due to its high market demand which commands relatively good farm prices for …show more content…
The most effective control measure to prevent the infestation of bruchid in large scale storage is chemical control (Daglishet et al., 1993), but it has advantages which are not environment and consumer benign such as increment in storage cost, chemical exposure to consumers and farmers, and development of resistance to the targeted pest (Gbaye et al., 2011). The efficiency of bio-control methods using plant materials or parasitoids are available but seems to be limited (Brisibe et al., 2011, Soundararajan et al., 2012). The most efficient and efficient control measure of bruchids which is environment and consumer friendly is host …show more content…
Extraction of DNA could be done from any samples but fresh samples are the best for extraction for good quality DNA. There are many protocols in extracting DNA which are simple, quick, less laborious and less time consuming which produce high quality and quantity of DNA e.g. QIAGEN RNeasy.
There are different types of molecular markers and they are grouped by Semagn et al., 2016 based on their method of analysis (Hybridization-based molecular markers or PCR-based molecular markers).
Molecular markers are also used to detect the genomic structure of different organisms, genotypic variations such as insertions, mutations, deletions and single nucleotide polymorphisms, genome organization and evolutions (Jones et al., 2009). In many crop breeding programmes, these markers are used to track loci and genomic regions which are strongly linked with a large number of agronomic and disease resistance traits found in crop species (Varshney et., 2007). In this study, we applied single nucleotide polymorphism (SNP) markers which are described in detail.
Single Nucleotide Polymorphism